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Título : Initiation of phage φ29 DNA replication by mutants with deletions at the carboxyl end of the terminal protein
Autor : Zaballos, Ángel, Salas, Margarita, Mellado, Rafael P.
Palabras clave : Recombinant DNA
Phage lambda PL promoter
Protein p3-dAMP complex
Fecha de publicación : 1986
Editor: Elsevier
Resumen: Series of deletions at the C end of p3, the phage φ29 DNA terminal protein (TP), have been constructed and characterized. Measurements of the activity of those deletion mutants in the formation of the p3-dAMP initiation complex in vitro indicate the need of an intact C-end for the normal TP primer function in DNA replication. It appears that the region at the C-end between aa 240 and 262 of p3, or part of it, might be essential for the normal TP function.
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ISSN: 0378-1119
DOI: 10.1016/0378-1119(86)90013-2
Citación : Gene 43(1-2): 103-110 (1986)
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