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Closed Access item Transcription Regulation in Bacillus subtilis Phage Φ29: Expression of the Viral Promoters throughout the Infection Cycle

Authors:Monsalve, María
Mario, Mencía
Rojo, Fernando
Salas, Margarita
Issue Date:20-Feb-1995
Citation:Virology 207(1): 84-96 (1980)
Abstract:Transcription of the genome of Bacillus subtilis phage Φ29 is tightly controlled, taking place in two stages, early and late. We have analyzed the abundance of the transcripts produced from each viral promoter throughout the infection cycle. We compare the relative strength of each promoter, as well as get a better understanding of the regulatory events, finding a new promoter regulated by the viral protein p4. The two strong early promoters, A2b and A2c, responsible for the expression of genes 6 to 1, are coordinately repressed by the viral protein p4, although repression is not complete; both promoters are still active at late times of infection. Since repression by protein p4 was very efficient in vitro, and affects its own synthesis, it is likely that this protein is produced in limiting amounts, not being bound to all viral DNA molecules present in the cell at a given time. Protein p4, also known to activate the late promoter responsible for the expression of all the structural end morphogenetic genes, is the key regulator of phage Φ29 development.
Publisher version (URL):http://dx.doi.org/10.1006/viro.1995.1048
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