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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/39135
Title: Assembly of the tail protein of the Bacillus subtilis phage φ29
Authors: García, Juan Antonio; Carrascosa, José L.; Salas, Margarita
Issue Date: Feb-1983
Publisher: Elsevier
Citation: Virology 125(1): 18-30 (1983)
Abstract: By in vitro complementation we have determined that gene 13 product functions during φ29 morphogenesis after the formation of 11− particles, specifically in the functional assembly of the tail protein, p9. Protein p9 from 8− but not from 8−13− extracts assembles in vitro into either 11−13− or 12−13− particles. The action of gene 13 product on p9 for its correct assembly has to take place in vivo; no complementation of 12−13− and 9− lysates occurs in vitro. Protein p9, isolated from φ29-infected cells, copurifies with the 13+ activity and it is present both in 13+ and 13− extracts as an aggregate with dimensions similar to those of the tail assembled in mature phage.
Publisher version (URL): http://dx.doi.org/10.1016/0042-6822(83)90060-0
URI: http://hdl.handle.net/10261/39135
DOI: 10.1016/0042-6822(83)90060-0
ISSN: 0042-6822
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