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Title: The protein covalently linked to the 5′ termini of the DNA of Bacillus subtilis phage θ29 is involved in the initiation of DNA replication
Authors: Mellado, Rafael P., Peñalva, Miguel Angel, Inciarte, M. R., Salas, Margarita
Issue Date: 15-Jul-1980
Publisher: Elsevier
Abstract: Bacillus subtilis was infected at 30° with θ29 mutants ts in each of four cistrons involved in phage DNA synthesis and, after a short pulse, the infected bacteria were shifted up to 42°C. Analysis of the labeled DNA, at different times after the shift-up, by alkaline sucrose gradient centrifugation showed that unit-length DNA was formed after infection with mutant ts3(132), suggesting a role of protein p3 in the initiation of replication. A similar result was obtained after ts2(98) infection. On the contrary, mutant ts5(17) did not produce unit-length phage DNA even at very late times after the shift-up, consistent with the possibility that this mutation affects an elongation (or maturation) process. Mutant ts6(1360) gave rise to unit-length DNA late after the shift-up suggesting that the mutation might affect an elongation or maturation process but that either it is leaky or that its function may be slowly replaced by a bacterial function. Infection of B. subtilis, under restrictive conditions, with sus mutants in cistron 3 produced unit-length θ29 DNA, both in neutral and alkaline sucrose gradients, suggesting that the parental protein itself present in the sus3 mutants is able to initiate the viral replication.
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ISSN: 0042-6822
???metadata.dc.identifier.doi???: 10.1016/0042-6822(80)90367-0
Citation: Virology 104(1): 84-96 (1980)
Appears in Collections:(CBM) Artículos
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