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Closed Access item The protein covalently linked to the 5′ termini of the DNA of Bacillus subtilis phage θ29 is involved in the initiation of DNA replication

Authors:Mellado, Rafael P.
Peñalva, Miguel Angel
Inciarte, M. R.
Salas, Margarita
Issue Date:15-Jul-1980
Publisher:Elsevier
Citation:Virology 104(1): 84-96 (1980)
Abstract:Bacillus subtilis was infected at 30° with θ29 mutants ts in each of four cistrons involved in phage DNA synthesis and, after a short pulse, the infected bacteria were shifted up to 42°C. Analysis of the labeled DNA, at different times after the shift-up, by alkaline sucrose gradient centrifugation showed that unit-length DNA was formed after infection with mutant ts3(132), suggesting a role of protein p3 in the initiation of replication. A similar result was obtained after ts2(98) infection. On the contrary, mutant ts5(17) did not produce unit-length phage DNA even at very late times after the shift-up, consistent with the possibility that this mutation affects an elongation (or maturation) process. Mutant ts6(1360) gave rise to unit-length DNA late after the shift-up suggesting that the mutation might affect an elongation or maturation process but that either it is leaky or that its function may be slowly replaced by a bacterial function. Infection of B. subtilis, under restrictive conditions, with sus mutants in cistron 3 produced unit-length θ29 DNA, both in neutral and alkaline sucrose gradients, suggesting that the parental protein itself present in the sus3 mutants is able to initiate the viral replication.
Publisher version (URL):http://dx.doi.org/10.1016/0042-6822(80)90367-0
URI:http://hdl.handle.net/10261/39082
ISSN:0042-6822
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