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Open Access item MALDI-TOF Mass Array Analysis of RASSF1A and SERPINB5 Methylation Patterns in Human Placenta and Plasma

Authors:Bellido, Maria Luz
Hmadcha, Abdelkrim
Holzgreve, Wolfgang
Keywords:DNA methylation, MALDI-TOF, Mass spectrometry, Placenta, Preeclampsia, Pregnancy
Issue Date:Apr-2010
Publisher:Society for the Study of Reproduction
Citation:Biology of Reproduction 82(4): 745-750 (2010)
Abstract:Differences in DNA methylation patterns between placenta and blood cells of pregnant women have been suggested as potential biomarkers for noninvasive prenatal diagnostic strategies, including for common obstetrical complications, such as preeclampsia. New findings in epigenetic origins of fetal or placental disorders may improve our ability for optimal management of these conditions. Using a novel high-throughput mass spectrometry on matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass array, we compared the quantitative methylation changes of RASSF1 and SERPINB5 (also known as MASPIN) genes in placenta and plasma samples. We analyzed the methylation status of a total of 3569 CpG dinucleotides on these two genes in 83 different samples: 50 plasma samples (20 from pregnant women and 30 from nonpregnant women) and 33 placenta tissue samples (25 from normal pregnancies and eight from preeclamptic pregnancies). The aim of this study was to assess the utility of epigenetic changes as biomarkers for noninvasive prenatal diagnostic procedures. Using a two-way hierarchical cluster analysis, significantly different methylation levels of the RASSF1 gene were found between placenta (normal and preeclamptic) and plasma samples of pregnant women. Although the SERPINB5 gene was hypomethylated in placenta DNA more than in plasma DNA, it did not demonstrate significant differences between studied groups. The MALDI-TOF mass spectrometry analysis of placenta and plasma DNA methylation patterns may serve as a tool for the study of gender-independent biomarkers in noninvasive prenatal diagnosis.
Description:6 páginas, 2 figuras, 2 tablas.-- et al.
Publisher version (URL):http://dx.doi.org/10.1095/biolreprod.109.082271
URI:http://hdl.handle.net/10261/39056
ISSN:0006-3363
E-ISSNmetadata.dc.identifier.doi = DOI:1529-7268
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