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Título : Substitution of the C-terminal domain of the Escherichia coli RNA polymerase α subunit by that from Bacillus subtilis makes the enzyme responsive to a Bacillus subtilis transcriptional activator1
Autor : Mencía, Mario, Monsalve, María, Rojo, Fernando, Salas, Margarita
Palabras clave : RNA polymerase
Transcription regulation
Fecha de publicación : 16-Jun-1998
Editor: Elsevier
Resumen: Regulatory protein p4 of Bacillus subtilis phage Φ29 activates transcription from the viral late A3 promoter by interacting with the C-terminal domain (CTD) of the B. subtilis RNA polymerase α subunit, thereby stabilizing the holoenzyme at the promoter. Protein p4 does not interact with the Escherichia coli RNA polymerase and cannot activate transcription with this enzyme. We have constructed a chimerical α subunit containing the N-terminal domain of the E. coli α subunit and the CTD of the B. subtilis α subunit. Reconstitution of RNA polymerases containing this chimerical α subunit, the E. coli β and β′ subunits, and the vegetative σ factor from either E. coli (σ70) or B. subtilis (σA), generated hybrid enzymes that were responsive to protein p4 and efficiently supported activation at the A3 promoter. Protein p4 activated transcription with the chimerical enzymes through the same activation surface used with B. subtilis RNA polymerase. Therefore, the B. subtilis α-CTD allowed activation by p4 even when the rest of the RNA polymerase subunits belonged to E. coli, a distantly related bacterium. These results strongly suggest that protein p4 works essentially by serving as an anchor that stabilizes RNA polymerase at the promoter.
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ISSN: 0022-2836
DOI: 10.1006/jmbi.1997.1463
Citación : Journal of Molecular Biology 275(2): 177-185 (1998)
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