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Título: | A reliable and simplified sj/β-TREC ratio quantification method for human thymic output measurement |
Autor: | Ferrando-Martínez, Sara; Franco, Jaime M. CSIC ORCID; Ruiz-Mateos, Ezequiel CSIC ORCID; Hernández, Ana; Ordóñez Fernández, Antonio CSIC ORCID; Gutiérrez-Carretero, Encarnación CSIC ORCID; Leal, Manuel CSIC | Palabras clave: | Sj-TREC DβJβ-TREC TREC ratio Intrathymic proliferation HIV Human thymic function |
Fecha de publicación: | 2010 | Editor: | Elsevier | Citación: | Journal of Immunological Methods 352(1-2): 111-117 (2010) | Resumen: | Current techniques to peripherally assess thymic function are: the signal-joint T-cell receptor excision circle (sj-TREC) level measurement and the naive T cell and CD31+ TREC-rich subset determination. However, all of them are indirect approaches and none could be considered a direct recent thymic emigrant (RTE) marker. To overcome their limitations, Dion et al. (2004) described the sj/β-TREC ratio that allows the peripheral quantification of the double negative to double positive intrathymic proliferation step. Nevertheless, the protocol described is expensive, sample and time-consuming, thus, limiting its usefulness. In this study, we describe a simplified protocol that reduces from 33 to 9 the amount of PCR reaction needed but maintaining the sensitivity and reproducibility of the original technique. In addition, we corroborated the effectiveness of our technique as an accurate thymic output-related marker by correlating the peripheral sj/β-TREC ratio with a direct measurement of thymic function as the percentage of double positive thymocytes (r = 0.601, p < 0.001). | Descripción: | 7 páginas, 3 figuras, 1 tabla. | Versión del editor: | http://dx.doi.org/10.1016/j.jim.2009.11.007 | URI: | http://hdl.handle.net/10261/38895 | DOI: | 10.1016/j.jim.2009.11.007 | ISSN: | 0022-1759 |
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