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Closed Access item Requirement for an A-tract structure at the binding sites of phage ø29 transcriptional activator

Authors:Nuez, Beatriz
Rojo, Fernando
Salas, Margarita
Keywords:A tract structure, DNA binding
Issue Date:24-Mar-1994
Citation:Journal of Molecular Biology 237(2): 175-181 (1994)
Abstract:The Bacillus subtilis phage φ29 transcriptional activator, protein p4, binds to the 5′-AACTTTTT-15 base-pair spacer-AAAATGTT-3′ inverted repeat. In this communication, we study the influence in protein p4 binding of the DNA helical structure within the protein p4 recognition sequences, 5′-AAAAATG-3′. Protein p4 could efficiently bind to a modified target in which the A-tracts had been changed into T-tracts (a different sequence with a similar structure). Binding was lost when the structure of the binding site was modified by an interrupting C residue. The results suggest that the DNA helical structure of the A-tracts is critical for p4 binding. Two models are described that would explain how protein p4 recognizes its target sequences on the DNA.
Publisher version (URL):http://dx.doi.org/10.1006/jmbi.1994.1219
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