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Closed Access item Isolation of nine nucler microsatellites in the common Mediterranean sea urchin, Paracentrotus lividus (Lamarck)

Authors:Calderón, I.
Turon, Xavier
Pascual, M.
Keywords:Sea urchin, Enrichment, Heterozygote deficit, Microsatellites, Paracentrotus
Issue Date:2009
Publisher:Blackwell Publishing
Citation:Molecular Ecology Resources 9(4) : 1145-1147 (2009)
Abstract:Nine polymorphic microsatellite loci were isolated and characterized for the edible common sea urchin Paracentrotus lividus. Loci were obtained from two genomic libraries enriched with different di-, tri- and tetranucleotides. Most microsatellites obtained were imperfect dinucleotides. Allelic variation was screened for a total of 56 individuals from two populations from North-Western Mediterranean. Microsatellites showed a number of alleles ranging from 13 to 35. Linkage disequilibrium was not detected between any pair of loci, and all loci showed a significant departure from Hardy–Weinberg equilibrium which is not likely to be the result of null alleles, suggesting that demographic features may be acting upon this commercially interesting species.
Description:3 Páginas ; 1 Tabla
Publisher version (URL):http://dx.doi.org/10.1111/j.1755-0998.2009.02585.x
Appears in Collections:(CEAB) Artículos

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