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Closed Access item Transition from initiation to elongation in the protein-primed ø29 DNA replication. Salt-dependent stimulation by the viral protein p6

Authors:Blanco, Luis
Bernad, Antonio
Salas, Margarita
Issue Date:1988
Publisher:American Society for Microbiology
Citation:Journal of Virology 62(11): 4167-4172 (1988)
Abstract:The transition step from the p3-dAMP initiation complex to the first elongated products, p3-(dAMP)2 and p3-(dAMP)3, requires a dATP concentration higher than that needed for the initiation reaction or for the further elongation of the p3-(dAMP)3 complex. The elongation in phi 29 DNA-protein p3 replication in vitro was strongly inhibited by salt. Under inhibitory salt concentration, the viral protein p6 greatly stimulated phi 29 DNA-protein p3 replication. The effect of protein p6 was not on the rate of elongation but on the amount of elongated product, stimulating the transition from initiation to formation of the first elongation products.
Publisher version (URL):http://jvi.asm.org/cgi/reprint/62/11/4167
URI:http://hdl.handle.net/10261/38628
ISSN:0022-538X
E-ISSNmetadata.dc.identifier.doi = DOI:1098-5514
Appears in Collections:(CBM) Artículos

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