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dc.contributor.authorGarcía, Pedro-
dc.contributor.authorHermoso, José Miguel-
dc.contributor.authorGarcía, Juan Antonio-
dc.contributor.authorGarcía, Ernesto-
dc.contributor.authorLópez, Rubens-
dc.contributor.authorSalas, Margarita-
dc.identifier.citationJournal of Virology 58(1): 31-35 (1986)es_ES
dc.description.abstractIncubation of extracts of Cp-1-infected Streptococcus pneumoniae with [alpha-32P]dATP produced a labeled treatment with micrococcal nuclease and sensitive to treatment with proteinase K. Incubation of the 32P-labeled protein with 5 M piperidine for 4 h at 50 degrees C released 5'-dAMP, indicating that a covalent complex between the terminal protein and 5'-dAMP was formed in vitro. When the four deoxynucleoside triphosphates were included in the reaction mixture, a labeled complex of slower electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels than the terminal protein-dAMP complex was also found, indicating that the Cp-1 terminal protein-dAMP complex can be elongated and, therefore, that it is an initiation complex. Treatment of the 32P-labeled terminal protein-dAMP complex with 5.8 M HCl at 110 degrees C for 2 h yielded phosphothreonine. These results, together with the resistance of the terminal protein-DNA linkage to hydroxylamine, suggest that the Cp-1 terminal protein is covalently linked to the DNA through a phosphoester bond between L-threonine and 5'-dAMP, namely, a O-5'-deoxyadenylyl-L-threonine bond.es_ES
dc.description.sponsorshipThis investigation was aided by Public Health Service research grant 5 R01 GM27242-05 from the National Institutes of Health, by grants 847 and 3325 from the Comisi6n Asesora para el Desarrollo de la Investigaci6n Cientifica y Tecnica, and by a grant from Fondo de Investigaciones Sanitarias. P.G. and J.A.G. were the recipients of a postdoctoral fellowship from the Spanish Research Council.es_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.titleFormation of a covalent complex between the terminal protein of pneumococcal bacteriophage Cp-1 and 5'-dAMPes_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderNational Institutes of Health (US)-
dc.contributor.funderComisión Asesora de Investigación Científica y Técnica, CAICYT (España)-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
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