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Title

Formation of a covalent complex between the terminal protein of pneumococcal bacteriophage Cp-1 and 5'-dAMP

AuthorsGarcía, Pedro ; Hermoso, José Miguel; García, Juan Antonio; García, Ernesto ; López, Rubens; Salas, Margarita
Issue DateApr-1986
PublisherAmerican Society for Microbiology
CitationJournal of Virology 58(1): 31-35 (1986)
AbstractIncubation of extracts of Cp-1-infected Streptococcus pneumoniae with [alpha-32P]dATP produced a labeled treatment with micrococcal nuclease and sensitive to treatment with proteinase K. Incubation of the 32P-labeled protein with 5 M piperidine for 4 h at 50 degrees C released 5'-dAMP, indicating that a covalent complex between the terminal protein and 5'-dAMP was formed in vitro. When the four deoxynucleoside triphosphates were included in the reaction mixture, a labeled complex of slower electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels than the terminal protein-dAMP complex was also found, indicating that the Cp-1 terminal protein-dAMP complex can be elongated and, therefore, that it is an initiation complex. Treatment of the 32P-labeled terminal protein-dAMP complex with 5.8 M HCl at 110 degrees C for 2 h yielded phosphothreonine. These results, together with the resistance of the terminal protein-DNA linkage to hydroxylamine, suggest that the Cp-1 terminal protein is covalently linked to the DNA through a phosphoester bond between L-threonine and 5'-dAMP, namely, a O-5'-deoxyadenylyl-L-threonine bond.
Publisher version (URL)http://jvi.asm.org/cgi/reprint/58/1/31
URIhttp://hdl.handle.net/10261/38613
ISSN0022-538X
E-ISSN1098-5514
Appears in Collections:(CBM) Artículos
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