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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/38611
Title: Adsorption of bacteriophage ø29 to Bacillus subtilis through the neck appendages of the viral particle
Authors: Villanueva, Nieves; Salas, Margarita
Issue Date: Apr-1981
Publisher: American Society for Microbiology
Citation: Journal of Virology 38(1): 15-19 (1981)
Abstract: Phage phi 29 particles produced under restrictive conditions by mutants in gene 12 have normal amounts of all of the structural proteins except the appendage protein, p12*, which is missing. These particles are not infective and do not adsorb to Bacillus subtilis cells. By in vitro complementation of 12- particles with extracts containing protein p12* or with purified protein p12*, the defective particles could bind the appendage protein and become infective and able to adsorb to bacteria. Therefore, the neck appendages of phage phi 29, formed by protein p12*, are involved in the interaction of the phage with the cell wall receptors. Protein p12*, purified in its native state, competed with wild-type phage for adsorption to bacteria. Also, protein p12* could displace adsorbed phage from bacteria. Since the displaced phage was infective, protein p12* does not seem to be modified after phage adsorption.
Publisher version (URL): http://jvi.asm.org/cgi/reprint/38/1/15
URI: http://hdl.handle.net/10261/38611
ISSN: 0022-538X
E-ISSN: 1098-5514
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