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Título : Genetic analysis of bacteriophage phi 29 of Bacillus subtilis: integration and mapping of reference mutants of two collections
Autor : Mellado, Rafael P., Moreno Muñoz, Francisco José, Viñuela, Eladio, Salas, Margarita, Reilly, B. E., Anderson, D. L.
Fecha de publicación : Aug-1976
Editor: American Society for Microbiology
Resumen: Reference mutants of Bacillus subtilis phage phi 29 of the Madrid and Minneapolis collections were employed to construct a genetic map. Suppressor-sensitive and temperature-sensitive mutants were assigned to 17 cistrons by quantitative complementation. Three-factor crosses were used to assign an unambiguous order for the 17 cistrons. Recombination frequencies determined by two-factor crosses were used to construct a linear genetic map of 24.4 recombination units. The genes were numbered sequentially from left to right (1 to 17) according to their relative map position.
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ISSN: 0022-538X
Citación : Journal of Virology 19(2): 495-500 (1976)
Appears in Collections:(CBM) Artículos

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