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Closed Access item Genetic analysis of bacteriophage phi 29 of Bacillus subtilis: integration and mapping of reference mutants of two collections

Authors:Mellado, Rafael P.
Moreno Muñoz, Francisco José
Viñuela, Eladio
Salas, Margarita
Reilly, B. E.
Anderson, D. L.
Issue Date:Aug-1976
Publisher:American Society for Microbiology
Citation:Journal of Virology 19(2): 495-500 (1976)
Abstract:Reference mutants of Bacillus subtilis phage phi 29 of the Madrid and Minneapolis collections were employed to construct a genetic map. Suppressor-sensitive and temperature-sensitive mutants were assigned to 17 cistrons by quantitative complementation. Three-factor crosses were used to assign an unambiguous order for the 17 cistrons. Recombination frequencies determined by two-factor crosses were used to construct a linear genetic map of 24.4 recombination units. The genes were numbered sequentially from left to right (1 to 17) according to their relative map position.
Publisher version (URL):http://jvi.asm.org/cgi/reprint/19/2/495
URI:http://hdl.handle.net/10261/38600
ISSN:0022-538X
E-ISSNmetadata.dc.identifier.doi = DOI:1098-5514
Appears in Collections:(CBM) Artículos

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