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|Title:||Genetic analysis of bacteriophage phi 29 of Bacillus subtilis: integration and mapping of reference mutants of two collections|
|Authors:||Mellado, Rafael P.; Moreno Muñoz, Francisco José; Viñuela, Eladio; Salas, Margarita; Reilly, Bernard E.; Anderson, D. L.|
|Publisher:||American Society for Microbiology|
|Citation:||Journal of Virology 19(2): 495-500 (1976)|
|Abstract:||Reference mutants of Bacillus subtilis phage phi 29 of the Madrid and Minneapolis collections were employed to construct a genetic map. Suppressor-sensitive and temperature-sensitive mutants were assigned to 17 cistrons by quantitative complementation. Three-factor crosses were used to assign an unambiguous order for the 17 cistrons. Recombination frequencies determined by two-factor crosses were used to construct a linear genetic map of 24.4 recombination units. The genes were numbered sequentially from left to right (1 to 17) according to their relative map position.|
|Publisher version (URL):||http://jvi.asm.org/cgi/reprint/19/2/495|
|Appears in Collections:||(CBM) Artículos|
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