Digital.CSIC > Biología y Biomedicina > Centro de Biología Molecular Severo Ochoa (CBM) > (CBM) Artículos >





Closed Access item A mutation in the C-terminal domain of the RNA polymerase alpha subunit that destabilizes the open complexes formed at the phage φ29 late A3 promoter

Authors:Calles, Belén
Monsalve, María
Rojo, Fernando
Salas, Margarita
Keywords:Transcription regulation, Protein-DNA interactions, RNA polymerase α subunit, Bacterial promoters, Transcription initiation
Issue Date:23-Mar-2001
Citation:Journal of Molecular Biology 307(1): 487-497 (2001)
Abstract:Regulatory protein p4 from Bacillus subtilis phage φ29 activates the viral late A3 promoter mainly by stabilizing the binding of RNA polymerase (RNAP) to it as a closed complex. This requires an interaction between protein p4 residue Arg120 and the C-terminal domain (CTD) of the RNAP α subunit. Several acidic residues of the α-CTD, considered as plausible targets for p4 residue Arg120, were individually changed into alanine. In addition, a truncated α subunit lacking the last four residues, two of which are acidic, was obtained. The modified α subunits were purified and reconstituted into RNAP holoenzyme in vitro. Protein p4 was found to be unable to activate the late A3 promoter when residue Glu297 of the α subunit was changed to Ala, a modification that did not impair transcription from several other promoters. Interestingly, protein p4 could stabilize the modified RNAP at the A3 promoter as a closed complex, although the open complexes formed were unstable and did not proceed to elongation complexes. Our results indicate that the change of the α residue Glu297 into Ala destabilizes the open complexes formed at this promoter, but not at other promoters. Considered in the context of earlier findings indicating that the RNAP α-CTD may participate in the transition from closed to intermediate complexes at some other promoters, the new results expand and clarify our view of its role in transcription initiation.
Publisher version (URL):http://dx.doi.org/10.1006/jmbi.2001.4511
Appears in Collections:(CBM) Artículos
(CNB) Artículos

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.