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Open Access item Analysis of Early Promoters of the Bacillus Bacteriophage GA-1

Authors:Horcajadas, José Antonio
Meijer, Wilhelmus J. J.
Rojo, Fernando
Salas, Margarita
Issue Date:Dec-2001
Publisher:American Society for Microbiology
Citation:Journal of Bacteriology 183(23): 6965-6970 (2001)
Abstract:Bacteriophage GA-1, which infects Bacillus sp. strain G1R, is evolutionarily related to phage 29, which infects Bacillus subtilis. We report the characterization of several GA-1 promoters located at either end of its linear genome. Some of them are unique for GA-1 and drive the expression of open reading frames that have no counterparts in the genome of 29 or related phages. These unique promoters are active at early infection times and are repressed at late times. In vitro transcription reactions revealed that the purified GA-1-encoded protein p6 represses the activity of these promoters, although the amount of p6 required to repress transcription was different for each promoter. The level of protein p6 produced in vivo increases rapidly during the first stage of the infection cycle. The protein p6 concentration may serve to modulate the expression of these early promoters as infection proceeds.
Publisher version (URL):http://dx.doi.org/10.1128/JB.183.23.6965-6970.2001
URI:http://hdl.handle.net/10261/38479
ISSN:6927-6932
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