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Title

A Postincision-Deficient TFIIH Causes Replication Fork Breakage and Uncovers Alternative Rad51- or Pol32-Mediated Restart Mechanisms

AuthorsMoriel-Carretero, María ; Aguilera, Andrés
Issue DateMar-2010
PublisherElsevier
CitationMolecular Cell 37(5): 690-701 (2010)
AbstractHomologous recombination is a major double-strand break (DSB) repair mechanism that acts during the S and G2 phases. In contrast, nucleotide excision repair (NER) is a major pathway for the repair of DNA bulky adducts that is unrelated to replication. We show that replication can be strongly disturbed in a specific type of rad3/XPD NER mutant of TFIIH, causing replication fork breakage. In contrast to classical NER-deficient mutations, the S. cerevisiae rad3-102 allele, which has a minimal impact on UV resistance, channels bulky adducts into DSBs. rad3-102 allows Rad1/XPF- and Rad2/XPG-catalyzed DNA incisions but fails to perform postincision steps retaining TFIIH at the damaged site. Broken forks are rescued by MRX-Rad52-Rfc1-dependent recombination via two types of replication restart mechanisms, one being Rad51 dependent and the other Pol32 dependent. Our results define the genetic and molecular hallmarks of replication fork breakage and restart and bring insights to understand specific NER-related human syndromes.
Description12 páginas, 7 figuras.
Publisher version (URL)http://dx.doi.org/10.1016/j.molcel.2010.02.008
URIhttp://hdl.handle.net/10261/38421
DOI10.1016/j.molcel.2010.02.008
ISSN1097-2765
Appears in Collections:(CABIMER) Artículos
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