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Purification in an active form of the phage ø29 protein p4 that controls the viral late transcription

AuthorsBarthelemy, Isabel; Lázaro, José M. ; Méndez Cormán, Enrique; Mellado, Rafael P.; Salas, Margarita
Issue Date12-Oct-1987
PublisherOxford University Press
CitationNucleic Acids Research 15(19 ) 7781-7793 (1987)
AbstractThe phage ø29 protein p4, that controls viral late transcription, was highly purified from Escherichia coli cells harbouring a gene 4-containing plasmid. This protein, representing about 6% of the total cellular protein, was obtained in a highly purified form. The protein was characterized as p4 by amino acid analysis and NH2—terminal sequence determination. The purified protein was active in an in vitro transcription assay, allowing specific initiation of transcription at the ø 29 A3 late promoter in the presence of Bacillus subtilis α43 -RNA polymerase holoenzyme.
Publisher version (URL)http://dx.doi.org/10.1093/nar/15.19.7781
Appears in Collections:(CBM) Artículos
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