Please use this identifier to cite or link to this item:
Título : Purification in an active form of the phage ø29 protein p4 that controls the viral late transcription
Autor : Barthelemy, Isabel, Lázaro, José M., Méndez, E., Mellado, Rafael P., Salas, Margarita
Fecha de publicación : 12-Oct-1987
Editor: Oxford University Press
Resumen: The phage ø29 protein p4, that controls viral late transcription, was highly purified from Escherichia coli cells harbouring a gene 4-containing plasmid. This protein, representing about 6% of the total cellular protein, was obtained in a highly purified form. The protein was characterized as p4 by amino acid analysis and NH2—terminal sequence determination. The purified protein was active in an in vitro transcription assay, allowing specific initiation of transcription at the ø 29 A3 late promoter in the presence of Bacillus subtilis α43 -RNA polymerase holoenzyme.
Versión del editor:
ISSN: 0305-1048
DOI: 10.1093/nar/15.19.7781
Citación : Nucleic Acids Research 15(19 ) 7781-7793 (1987)
Appears in Collections:(CBM) Artículos

Files in This Item:
File Description SizeFormat 
referencia119.pdf1,6 MBAdobe PDFView/Open
Show full item record

Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.