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http://hdl.handle.net/10261/38144
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Mendieta, Jesús | - |
dc.contributor.author | Pérez-Lago, Laura | - |
dc.contributor.author | Salas, Margarita | - |
dc.contributor.author | Camacho, Ana | - |
dc.date.accessioned | 2011-07-30T07:44:54Z | - |
dc.date.available | 2011-07-30T07:44:54Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Nucleic Acids Research 35 (10): 3252-3261 (2007) | es_ES |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10261/38144 | - |
dc.description.abstract | The bacteriophage Ø29 transcriptional regulator p4 binds to promoters of different intrinsic activities. The p4–DNA complex contains two identical protomers that make similar interactions with the target sequence 5′-AACTTTTT-15 bp-AAAATGTT-3′. To define how the various elements in the target sequence contribute to p4's affinity, we studied p4 binding to a series of mutated binding sites. The binding specificity depends critically on base pairs of the target sequence through both direct as well as indirect readout. There is only one specific contact between a base and an amino acid residue; other contacts take place with the phosphate backbone. Alteration of direct amino acid–base contacts, or mutation of non-contacted A·T base pairs at A-tracts abolished binding. We generated three 5 ns molecular dynamics (MD) simulations to investigate the basis for the p4–DNA complex specificity. Recognition is controlled by the protein and depends on DNA dynamic properties. MD results on protein–DNA contacts and the divergence of p4 affinity to modified binding sites reveal an inherent asymmetry, which is required for p4-specific binding and may be crucial for transcription regulation. | es_ES |
dc.description.sponsorship | This study was supported by the Ministerio de Educación y Ciencia of Spain (Grant BFU-2005-0733 to M.S.), the Comunidad de Madrid (Grant 08.2/0026.1/2005 to A.C.; Grant S-0505/MAT-0283 to M.S. and a fellowship to L.P.-L.) and an Institutional Grant from Fundación Ramón Areces to the Centro de Biología Molecular ‘Severo Ochoa’.Funding to pay the Open Access publication charge was provided by was provided by the Ministerio de Educatión y Ciencia. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.rights | openAccess | es_ES |
dc.title | DNA sequence-specific recognition by a transcriptional regulator requires indirect readout of A-tracts | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1093/nar/gkm180 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1093/nar/gkm180 | es_ES |
dc.identifier.e-issn | 1362-4962 | - |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.contributor.funder | Fundación Ramón Areces | - |
dc.identifier.funder | http://dx.doi.org/10.13039/100008054 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.pmid | 17452358 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
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