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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Hermoso, José Miguel | - |
dc.contributor.author | Méndez Cormán, Enrique | - |
dc.contributor.author | Soriano, Fernando | - |
dc.contributor.author | Salas, Margarita | - |
dc.date.accessioned | 2011-07-28T12:45:12Z | - |
dc.date.available | 2011-07-28T12:45:12Z | - |
dc.date.issued | 1985 | - |
dc.identifier.citation | Nucleic Acids Research 13(21): 7715-7728 (1985) | es_ES |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10261/38103 | - |
dc.description.abstract | B. subtilis phage φ29 has a terminal protein, p3, covalently linked to the 5'ends of the DNA through a phosphodiester bond between a serine residue and 5'-dAMP. This protein acts as a primer in DNA replication by forming an initiation complex with the 5'-terminal nucleotide dAMP. The amino acid sequence of the terminal protein, deduced from the nucleotide sequence of gene 3, showed the presence of 18 serine residues in a total of 266 amino acids. In this paper we have identified the serine involved in the linkage with the DNA as the residue 232, located close to the C-terminus of the molecule. This result was obtained by amino acid analysis of the peptide that remains linked to the DNA after proteinase K digestion of the terminal protein-φ29 DNA complex and automated Edman degradation of the corresponding {125I}-labeled tryptic peptide. Prediction of the secondary structure of the terminal protein suggested that the serine residue involved in the linkage with the DNA is placed in a β-turn, probably located on the external part of the molecule, as indicated by hydropathic values. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.rights | openAccess | es_ES |
dc.title | Location of the serine residue involved in the linkage between the terminal protein and the DNA of phage ø29 | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1093/nar/13.21.7715 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1093/nar/13.21.7715 | es_ES |
dc.identifier.pmid | 3934646 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
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referencia100.pdf | 1,3 MB | Adobe PDF | Visualizar/Abrir |
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