DSpace

Digital.CSIC > Biología y Biomedicina > Centro de Biología Molecular Severo Ochoa (CBM) > (CBM) Artículos >

Share

EndNote

Impact

Open Access item Location of the serine residue involved in the linkage between the terminal protein and the DNA of phage ø29

Authors:Hermoso, José Miguel
Méndez, E.
Soriano, F.
Salas, Margarita
Issue Date:1985
Publisher:Oxford University Press
Citation:Nucleic Acids Research 13(21): 7715-7728 (1985)
Abstract:B. subtilis phage φ29 has a terminal protein, p3, covalently linked to the 5'ends of the DNA through a phosphodiester bond between a serine residue and 5'-dAMP. This protein acts as a primer in DNA replication by forming an initiation complex with the 5'-terminal nucleotide dAMP. The amino acid sequence of the terminal protein, deduced from the nucleotide sequence of gene 3, showed the presence of 18 serine residues in a total of 266 amino acids. In this paper we have identified the serine involved in the linkage with the DNA as the residue 232, located close to the C-terminus of the molecule. This result was obtained by amino acid analysis of the peptide that remains linked to the DNA after proteinase K digestion of the terminal protein-φ29 DNA complex and automated Edman degradation of the corresponding {125I}-labeled tryptic peptide. Prediction of the secondary structure of the terminal protein suggested that the serine residue involved in the linkage with the DNA is placed in a β-turn, probably located on the external part of the molecule, as indicated by hydropathic values.
Publisher version (URL):http://dx.doi.org/10.1093/nar/13.21.7715
URI:http://hdl.handle.net/10261/38103
ISSN:0305-1048
???metadata.dc.identifier.doi???:10.1093/nar/13.21.7715
Appears in Collections:(CBM) Artículos

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.