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Open Access item Initiation of phage ø29 DNA replication by the terminal protein modified at the carboxyl end

Authors:Mellado, Rafael P.
Salas, Margarita
Issue Date:1983
Publisher:Oxford University Press
Citation:Nucleic Acids Research 11(21): 7397-7407 (1983)
Abstract:A mutant at the carboxyl end of the terminal protein, p3, of phage π29 DNA has been constructed by inserting an oligonucleo-tide containing the stop translation codon TGA in the three possible reading frames, immediately downstream of a π29 DNA fragment coding for all but the last five amino acids of protein p3. The activity in the formation of the p3-dAMP initiation complex in vitro of this mutant as well as another one previously isolated, also mutated at the carboxyl end, have been tested. The results obtained suggest that an intact carboxyl end in the π29 terminal protein is essential for its normal primer function in DNA replication.
Publisher version (URL):http://dx.doi.org/10.1093/nar/11.21.7397
URI:http://hdl.handle.net/10261/38096
ISSN:0305-1048
10.1093/nar/11.21.7397
Appears in Collections:(CBM) Artículos

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