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Open Access item Comparison of the A-T rich regions and the Bacillus subtilis RNA polymerase binding sites in phage ø29

Authors:Sogo, J. M.
Rodeño, P.
Koller, Th
Viñuela, Eladio
Salas, Margarita
Issue Date:1979
Publisher:Oxford University Press
Citation:Nucleic Acids Research 7, 107-120
Abstract:By using a modification of the BAC spreading method for mounting the DNA for electron microscopy, partial denaturation maps of protein-free ø29 DNA and of ø29 DNA containing protein p3 were obtained. In ø29 P3-DNA1 the protein does not seem to influence the melting of the ends of the molecules. The comparison of the partial denaturation map and the B. subtilis RNA polymerase binding sites indicates that five of the seven early promoters (Al, A2, A3, B2 and C2) are located in A-T rich DNA regions whereas the other two early promoters (Bl and Cl) are located in less A-T rich sites.
Publisher version (URL):http://dx.doi.org/10.1093/nar/7.1.107
URI:http://hdl.handle.net/10261/38089
ISSN:0305-1048
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Appears in Collections:(CBM) Artículos

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