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Title: | Purification and Properties of DNA-Dependent RNA Polymerase from Bacillus subtilis Vegetative Cells |
Authors: | Ávila, Jesús CSIC ORCID ; Hermoso, José Miguel; Viñuela, Eladio; Salas, Margarita CSIC ORCID | Issue Date: | Aug-1971 | Publisher: | Wiley-Blackwell | Citation: | European Journal of Biochemistry 21(4): 526-535 (1971) | Abstract: | A purification procedure to prepare highly purified DNA-dependent RNA polymerase from Bacillus subtilis vegetative cells is described. The enzyme consists of four different subunits, β′, β, σ and α (molecular weights 154000 for β′ and β, 56000 for σ and 43000 for α) in a molar ratio 1:1:1:2. By phosphocellulose chromatography RNA polymerase has been disscociated in σ subunit and core enzyme, containing β′, β and α subunits in a molar ratio 1:1:2; the σ subunit stimulates the activity of core polymerase with ϕ 29 DNA but not with poly[d(A—T)]. The general properties of the enzyme are also described. | Publisher version (URL): | http://dx.doi.org/10.1111/j.1432-1033.1971.tb01498.x | URI: | http://hdl.handle.net/10261/38086 | DOI: | 10.1111/j.1432-1033.1971.tb01498.x | ISSN: | 0014-2956 |
Appears in Collections: | (CBM) Artículos |
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j.1432-1033.1971.tb01498.x.pdf | 1,01 MB | Adobe PDF | View/Open |
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