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Open Access item Purification and Properties of DNA-Dependent RNA Polymerase from Bacillus subtilis Vegetative Cells

Authors:Avila, Jesús
Hermoso, José Miguel
Viñuela, Eladio
Salas, Margarita
Issue Date:Aug-1971
Citation:European Journal of Biochemistry 21(4): 526-535 (1971)
Abstract:A purification procedure to prepare highly purified DNA-dependent RNA polymerase from Bacillus subtilis vegetative cells is described. The enzyme consists of four different subunits, β′, β, σ and α (molecular weights 154000 for β′ and β, 56000 for σ and 43000 for α) in a molar ratio 1:1:1:2. By phosphocellulose chromatography RNA polymerase has been disscociated in σ subunit and core enzyme, containing β′, β and α subunits in a molar ratio 1:1:2; the σ subunit stimulates the activity of core polymerase with ϕ 29 DNA but not with poly[d(A—T)]. The general properties of the enzyme are also described.
Publisher version (URL):http://dx.doi.org/10.1111/j.1432-1033.1971.tb01498.x
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