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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/38078
Title: Isolation of a strong suppressor of nonsense mutations in Bacillus subtilis
Authors: Mellado, Rafael P.; Viñuela, Eladio; Salas, Margarita
Issue Date: 1976
Publisher: Wiley-Blackwell
Citation: European Journal of Biochemistry 65(1): 213-223 (1976)
Abstract: By treatment of Bacillus subtilis MO-101-P spoA− met thr− su− with ethyl methanesulfonate, a strong suppressor strain of nonsense mutations, B. subtilis MO-101-P spoA− [met−]+ thr− su+44 was isolated. This strain does not suppress phage φ 29 mutant susB47, selected on a B. subtilis strain containing the su+3 suppressor isolated by Georgopoulos. A revertant from this mutant, susB610, was isolated, being suppressed by both the su+3 and su+44 suppressor strains. The efficiency of suppression by strain su+44 is about 50%. The experiments shown in this paper suggest that strain su+44 contains an amber and strain su+3 an ochre suppressor.
Publisher version (URL): http://dx.doi.org/10.1111/j.1432-1033.1976.tb10408.x
URI: http://hdl.handle.net/10261/38078
DOI: 10.1111/j.1432-1033.1976.tb10408.x
ISSN: 0014-2956
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