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Open Access item Isolation of a strong suppressor of nonsense mutations in Bacillus subtilis

Authors:Mellado, Rafael P.
Viñuela, Eladio
Salas, Margarita
Issue Date:1976
Citation:European Journal of Biochemistry 65(1): 213-223 (1976)
Abstract:By treatment of Bacillus subtilis MO-101-P spoA− met thr− su− with ethyl methanesulfonate, a strong suppressor strain of nonsense mutations, B. subtilis MO-101-P spoA− [met−]+ thr− su+44 was isolated. This strain does not suppress phage φ 29 mutant susB47, selected on a B. subtilis strain containing the su+3 suppressor isolated by Georgopoulos. A revertant from this mutant, susB610, was isolated, being suppressed by both the su+3 and su+44 suppressor strains. The efficiency of suppression by strain su+44 is about 50%. The experiments shown in this paper suggest that strain su+44 contains an amber and strain su+3 an ochre suppressor.
Publisher version (URL):http://dx.doi.org/10.1111/j.1432-1033.1976.tb10408.x
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