Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/38059
Share/Impact:
Título : Assembly of B. subtilis phage ø29. I. Mutants in the cistrons coding for the structural proteins
Autor : Camacho, Ana Gema, Jiménez Diaz-Benjumea, Fernando, Torre, J. de la, Carrascosa, José L., Mellado, Rafael P., Vázquez Calvo, M.ª Carmen, Viñuela, Eladio, Salas, Margarita
Fecha de publicación : 1977
Editor: Wiley-Blackwell
Resumen: The effect on phage morphogenesis of sus mutations in the cistrons coding for nonstructural proteins has been studied. Mutants in three cistrons analyzed that are involved in phage DNA synthesis, as well as in cistron 16 which codes for a late nonstructural protein, produce prolate capsids which are more rounded at the corners than complete phage heads and have an internal core; they contain the head proteins, the upper collar protein and protein p7, not present in mature phage particles. Mutants in cistron 7 do not produce capsids nor other phage-related structures; this result and the presence of p7 in phage capsids suggest an essential role in capsid assembly for this protein. The protein product of cistron 13 is probably needed for a stable DNA encapsulation since mutants in this cistron produce mainly DNA-free complete phage particles and only about 10% of uninfective DNA-containing complete phage. Cistron 15 codes for a late, partially dispensable, nonstructural protein which is present in the DNA-free capsids produced after infection with the delayed-lysis mutant sus14(1242), used as the wild-type control, or with mutants in cistrons 9, 11, 12 and 13. Proteins p15 and p16 are probably involved in the encapsulation of viral DNA in a prohead.
Versión del editor: http://dx.doi.org/10.1111/j.1432-1033.1977.tb11291.x
URI : http://hdl.handle.net/10261/38059
ISSN: 0014-2956
DOI: 10.1111/j.1432-1033.1977.tb11291.x
Citación : European Journal of Biochemistry 73(1): 57-72 (1977)
Appears in Collections:(CBM) Artículos

Files in This Item:
File Description SizeFormat 
j.1432-1033.1977.tb11291.x.pdf3,26 MBAdobe PDFView/Open
Show full item record
 
CSIC SFX LinksSFX Query


Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.