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Closed Access item Physical map of bacteriophage ø29

Authors:Inciarte, M. R.
Lázaro, José M.
Salas, Margarita
Viñuela, Eladio
Issue Date:15-Oct-1976
Publisher:Elsevier
Citation:Virology 74(2): 314-323 (1976)
Abstract:Restriction endonuclease EcoRI cleaves linear 29 DNA at four points yielding five fragments (A–E) of molecular weights 6.0 × 106, 3.8 × 106, 1.3 × 106, 0.6 × 106, and 0.3 × 106. The relative order of the fragments was shown to be A, B, E, D, C by analysis of the EcoRI partial digestion products and from the study of the fragments obtained by treatment of protein-containing DNA with EcoRI and trypsin. The EcoRI cleavage map of 29 DNA has been ordered relative to the genetic map by marker rescue experiments.
Publisher version (URL):http://dx.doi.org/10.1016/0042-6822(76)90338-X
URI:http://hdl.handle.net/10261/38024
ISSN:0042-6822
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