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Título: | Solution NMR structure of a human FGF-1 monomer, activated by a hexasaccharide heparin-analogue |
Autor: | Canales, Ángeles CSIC ORCID; Lozano, R.M. CSIC ORCID ; López-Méndez, Blanca CSIC; Angulo, Jesús CSIC ORCID ; Ojeda, Rafael; Nieto, Pedro M. CSIC ORCID ; Martín-Lomas, Manuel CSIC; Giménez-Gallego, Guillermo CSIC; Jiménez-Barbero, Jesús CSIC ORCID | Palabras clave: | Fibroblas growth factor Heparin-like hexasaccharide Protein-carbohydrate complex |
Fecha de publicación: | 21-sep-2006 | Editor: | John Wiley & Sons | Citación: | FEBS Journal 273(20): 4716-4727 (2006) | Resumen: | The 3D structure of a complex formed by the acidic fibroblast growth factor (FGF-1) and a specifically designed synthetic heparin hexasaccharide has been determined by NMR spectroscopy. This hexasaccharide can substitute natural heparins in FGF-1 mitogenesis assays, in spite of not inducing any apparent dimerization of the growth factor. The use of this well defined synthetic heparin analogue has allowed us to perform a detailed NMR structural analysis of the heparin–FGF interaction, overcoming the limitations of NMR to deal with the high molecular mass and heterogeneity of the FGF-1 oligomers formed in the presence of natural heparin fragments. Our results confirm that glycosaminoglycans induced FGF-1 dimerization either in a cis or trans disposition with respect to the heparin chain is not an absolute requirement for biological activity. | Descripción: | 12 páginas, 7 figuras, 2 tablas. | Versión del editor: | http://dx.doi.org/10.1111/j.1742-4658.2006.05474.x | URI: | http://hdl.handle.net/10261/37894 | DOI: | 10.1111/j.1742-4658.2006.05474.x | ISSN: | 1742-464X |
Aparece en las colecciones: | (CIB) Artículos (IIQ) Artículos |
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