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Open Access item Molecular Interactions and Protein-Induced DNA Hairpin in the Transcriptional Control of Bacteriophage Ø29 DNA

Authors:Camacho, Ana Gema
Salas, Margarita
Keywords:Transcription regulator, Nucleoprotein complex
Issue Date:13-Dec-2010
Publisher:Multidisciplinary Digital Publishing Institute
Citation:International Journal of Molecular Sciences 11(12): 5129-5142 (2010)
Abstract:Studies on the regulation of phage Ø29 gene expression revealed a new mechanism to accomplish simultaneous activation and repression of transcription leading to orderly gene expression. Two phage-encoded early proteins, p4 and p6, bind synergistically to DNA, modifying the topology of the sequences encompassing early promoters A2c and A2b and late promoter A3 in a hairpin that allows the switch from early to late transcription. Protein p6 is a nucleoid-like protein that binds DNA in a non-sequence specific manner. Protein p4 is a sequence-specific DNA binding protein with multifaceted sequence-readout properties. The protein recognizes the chemical signature of only one DNA base on the inverted repeat of its target sequence through a direct-readout mechanism. In addition, p4 specific binding depends on the recognition of three A-tracts by indirect-readout mechanisms. The biological importance of those three A-tracts resides in their individual properties rather than in the global curvature that they may induce.
Publisher version (URL):http://dx.doi.org/10.3390/ijms1112512
Appears in Collections:(CBM) Artículos

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