English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/37826
Title: A passion for research
Other Titles: Memoires a senior scientist
Authors: Salas, Margarita
Issue Date: 2009
Publisher: Springer
Citation: Cellular and Molecular Life Sciences 66(24): 3827-3830
Abstract: During my postdoctoral training in Severo Ochoa’s laboratory, I determined the direction of reading of the genetic message and I discovered two proteins in Escherichia coli involved in the initiation of protein synthesis. After my return to Spain, I have been working with the Bacillus subtilis phage ϕ29. We discovered a protein covalently linked to the 5′ DNA ends that is the primer for the initiation of ϕ29 DNA replication. We also found that the phage-encoded DNA polymerase has unique properties such as processivity and strand displacement activity. These properties, in addition to its high fidelity, have made the ϕ29 DNA polymerase the ideal enzyme for DNA amplification, both for rolling circle and whole-genome amplification. I am happy to say that the work carried out in my laboratory has been possible thanks to many brilliant students and collaborators, most of whom have become high quality independent scientists.
Publisher version (URL): http://dx.doi.org/10.1007/s00018-009-0175-3
URI: http://hdl.handle.net/10261/37826
DOI: 10.1007/s00018-009-0175-3
ISSN: 1420-682X
Appears in Collections:(CBM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.