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Closed Access item A passion for research

Other Titles:Memoires a senior scientist
Authors:Salas, Margarita
Issue Date:2009
Citation:Cellular and Molecular Life Sciences 66(24): 3827-3830
Abstract:During my postdoctoral training in Severo Ochoa’s laboratory, I determined the direction of reading of the genetic message and I discovered two proteins in Escherichia coli involved in the initiation of protein synthesis. After my return to Spain, I have been working with the Bacillus subtilis phage ϕ29. We discovered a protein covalently linked to the 5′ DNA ends that is the primer for the initiation of ϕ29 DNA replication. We also found that the phage-encoded DNA polymerase has unique properties such as processivity and strand displacement activity. These properties, in addition to its high fidelity, have made the ϕ29 DNA polymerase the ideal enzyme for DNA amplification, both for rolling circle and whole-genome amplification. I am happy to say that the work carried out in my laboratory has been possible thanks to many brilliant students and collaborators, most of whom have become high quality independent scientists.
Publisher version (URL):http://dx.doi.org/10.1007/s00018-009-0175-3
Appears in Collections:(CBM) Artículos

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