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| Título: | The Dictyostelium discoideum acaA gene Is transcribed from alternative promoters during aggregation and multicellular development |
Autor: | Galardi-Castilla, María CSIC ORCID; Garciandía, Ane CSIC; Suárez, Teresa CSIC ORCID ; Sastre, Leandro CSIC ORCID | Fecha de publicación: | 11-oct-2010 | Editor: | Public Library of Science | Citación: | PLoS ONE 5(10): e13286 (2010) | Descripción: | [Background]: Extracellular cAMP is a key extracellular signaling molecule that regulates aggregation, cell differentiation and morphogenesis during multi-cellular development of the social amoeba Dictyostelium discoideum. This molecule is produced by three different adenylyl cyclases, encoded by the genes acaA, acrA and acgA, expressed at different stages of development and in different structures. [Methodology/Principal Findings]: This article describes the characterization of the promoter region of the acaA gene, showing that it is transcribed from three different alternative promoters. The distal promoter, promoter 1, is active during the aggregation process while the more proximal promoters are active in tip-organiser and posterior regions of the structures. A DNA fragment containing the three promoters drove expression to these same regions and similar results were obtained by in situ hybridization. Analyses of mRNA expression by quantitative RT-PCR with specific primers for each of the three transcripts also demonstrated their different temporal patterns of expression. [Conclusions/Significance]: The existence of an aggregation-specific promoter can be associated with the use of cAMP as chemo-attractant molecule, which is specific for some Dictyostelium species. Expression at late developmental stages indicates that adenylyl cyclase A might play a more important role in post-aggregative development than previously considered. | Versión del editor: | http://dx.doi.org/10.1371/journal.pone.0013286 | URI: | http://hdl.handle.net/10261/37819 | DOI: | 10.1371/journal.pone.0013286 | ISSN: | 1932-6203 |
| Aparece en las colecciones: | (IIBM) Artículos |
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| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| Plosone 2010 vol5 n10 ppe13286.pdf | 12,34 MB | Adobe PDF |  Visualizar/Abrir |
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