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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/37777
Title: Osmotin, a plant antifungal protein, subverts signal transduction to enhance fungal cell susceptibility.
Authors: Yun, Dae-Jin; Ibeas, José I.; Lee, Hyeseung; Coca, María; Narasimhan, Meena L.; Uesono, Yukifumi; Hasegawa, Paul M.; Pardo, José M.; Bressan, Ray A.
Issue Date: May-1998
Publisher: Elsevier
Citation: Molecular Cell 1(6): 807-817 (1998).
Abstract: The plant pathogenesis-related protein osmotin is an antifungal cytotoxic agent that causes rapid cell death in the yeast S. cerevisiae. We show here that osmotin uses a signal transduction pathway to weaken defensive cell wall barriers and increase its cytotoxic efficacy. The pathway activated by osmotin includes the regulatory elements of the mating pheromone response STE4, STE18, STE20, STE5, STE11, STE7, FUS3, KSS1, and STE12. Neither the pheromone receptor nor its associated G protein α subunit GPA1 are required for osmotin action. However, mutation of SST2, a negative regulator of Gα proteins, resulted in supersensitivity to osmotin. Phosphorylation of STE7 was rapidly stimulated by osmotin preceding any changes in cell vitality or morphology. These results demonstrate that osmotin subverts target cell signal transduction as part of its mechanism of action.
Description: 11 pages, 2 tablas, 7 figures, 70 references. We are deeply indebted to Henrik Dohlman, Malcolm Whiteway, Michael Gustin, Kunihiro Matsumoto, Ekkehard Leberer, Gerald Fink, and Beverly Errede for providing plasmids; Bradley R. Cairns for STE7 antibody; Kendall J. Blumer for yeast strains; and Jean Clithero for technical assistance.
Publisher version (URL): doi:10.1016/S1097-2765(00)80080-5
URI: http://hdl.handle.net/10261/37777
ISSN: 1097-2765
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