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Closed Access item Spo0A, the key transcriptional regulator for entrance into sporulation, is an inhibitor of DNA replication
Meijer, Wilhelmus J. J.
|Keywords:||B. subtilis, Spo0A|
|Publisher:||Nature Publishing Group|
|Citation:||Embo Journal 25: 3890 - 3899 (2006)|
|Abstract:||The transcription factor Spo0A is a master regulator for entry into sporulation in Bacillus subtilis and also regulates expression of the virulent B. subtilis phage 29. Here, we describe a novel function for Spo0A, being an inhibitor of DNA replication of both, the 29 genome and the B. subtilis chromosome. Binding of Spo0A near the 29 DNA ends, constituting the two origins of replication of the linear 29 genome, prevents formation of 29 protein p6-nucleoprotein initiation complex resulting in inhibition of 29 DNA replication. At the B. subtilis oriC, binding of Spo0A to specific sequences, which mostly coincide with DnaA-binding sites, prevents open complex formation. Thus, by binding to the origins of replication, Spo0A prevents the initiation step of DNA replication of either genome. The implications of this novel role of Spo0A for phage 29 development and the bacterial chromosome replication during the onset of sporulation are discussed.|
|Publisher version (URL):||http://dx.doi.org/10.1038/sj.emboj.7601266|
|Appears in Collections:||(CBM) Artículos|
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