Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/37754
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A uracil-DNA glycosylase inhibitor encoded by a non-uracil containing viral DNA |
Autor: | Serrano-Heras, Gemma CSIC ORCID; Salas, Margarita CSIC ORCID ; Bravo, Alicia CSIC ORCID | Fecha de publicación: | 18-ene-2006 | Editor: | American Society for Biochemistry and Molecular Biology | Citación: | Journal of Biological Chemistry 281(11): 7068-7074 (2006) | Resumen: | Uracil-DNA glycosylase (UDG) is an enzyme involved in the base excision repair pathway. It specifically removes uracil from both single-stranded and double-stranded DNA. The genome of the Bacillus subtilis phage ϕ29 is a linear double-stranded DNA with a terminal protein covalently linked at each 5′-end. Replication of ϕ29 DNA starts by a protein-priming mechanism and generates intermediates that have long stretches of single-stranded DNA. By using in vivo chemical cross-linking and affinity chromatography techniques, we found that UDG is a cellular target for the early viral protein p56. Addition of purified protein p56 to B. subtilis extracts inhibited the endogenous UDG activity. Moreover, extracts from ϕ29-infected cells were deficient in UDG activity. We suggested that inhibition of the cellular UDG is a defense mechanism developed by ϕ29 to prevent the action of the base excision repair pathway if uracil residues arise in their replicative intermediates. Protein p56 is the first example of a UDG inhibitor encoded by a non-uracil-containing viral DNA. | Versión del editor: | http://dx.doi.org/10.1074/jbc.M511152200 | URI: | http://hdl.handle.net/10261/37754 | DOI: | 10.1074/jbc.M511152200 | ISSN: | 0021-9258 |
Aparece en las colecciones: | (CBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
uracil-DNA_Serrano.pdf | 297,81 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
22
checked on 07-mar-2024
WEB OF SCIENCETM
Citations
23
checked on 27-feb-2024
Page view(s)
319
checked on 18-mar-2024
Download(s)
221
checked on 18-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.