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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/37715
Title: A new plasmid vector for regulated gene expression in Bacillus subtilis
Authors: Serrano-Heras, Gemma; Salas, Margarita; Bravo García, Alicia
Keywords: Bacillus subtilis
Plasmid vector
Regulated gene expression
Issue Date: Nov-2005
Publisher: Elsevier
Citation: Plasmid 54(3): 278-282 (2005)
Abstract: We have developed a novel regulated expression vector for Bacillus subtilis based on the Staphylococcus aureus plasmid pUB110. This vector, named pPR54, carries the PR promoter and the cI857 gene (encoding a temperature-sensitive transcriptional repressor) from the Escherichia coli phage λ. Using the gfp gene from the jellyfish Aequorea victoria as a reporter, we show that pPR54 is a useful vector for controllable production of heterologous proteins in B. subtilis.
Publisher version (URL): http://dx.doi.org/10.1016/j.plasmid.2005.04.002
URI: http://hdl.handle.net/10261/37715
ISSN: 0147-619X
DOI: 10.1016/j.plasmid.2005.04.002
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