DSpace

Digital.CSIC > Biología y Biomedicina > Centro de Biología Molecular Severo Ochoa (CBM) > (CBM) Artículos >

Share

EndNote

Impact

Links

Closed Access item A new plasmid vector for regulated gene expression in Bacillus subtilis

Authors:Serrano-Heras, Gemma
Salas, Margarita
Bravo García, Alicia
Keywords:Bacillus subtilis, Plasmid vector, Regulated gene expression
Issue Date:Nov-2005
Publisher:Elsevier
Citation:Plasmid 54(3): 278-282 (2005)
Abstract:We have developed a novel regulated expression vector for Bacillus subtilis based on the Staphylococcus aureus plasmid pUB110. This vector, named pPR54, carries the PR promoter and the cI857 gene (encoding a temperature-sensitive transcriptional repressor) from the Escherichia coli phage λ. Using the gfp gene from the jellyfish Aequorea victoria as a reporter, we show that pPR54 is a useful vector for controllable production of heterologous proteins in B. subtilis.
Publisher version (URL):http://dx.doi.org/10.1016/j.plasmid.2005.04.002
URI:http://hdl.handle.net/10261/37715
ISSN:0147-619X
???metadata.dc.identifier.doi???:10.1016/j.plasmid.2005.04.002
Appears in Collections:(CBM) Artículos

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.