English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/37668
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

A spectroscopy approach to the study of virus infection in the endophytic fungus Epichloe festucae

AuthorsPetisco, Cristina ; García Criado, Balbino ; Zabalgogeazcoa, Iñigo ; Vázquez de Aldana, Beatriz R. ; García Ciudad, Antonia
Issue Date8-Jun-2011
PublisherBioMed Central
CitationVirology Journal. 8(1):286 (2011)
AbstractAbstract Background In this work we propose a rapid method based on visible and near-infrared (Vis-NIR) spectroscopy to determine the occurrence of double-stranded RNA (dsRNA) viruses in Epichloë festucae strains isolated from Festuca rubra plants. In addition, we examined the incidence of infections by E. festucae in populations of F. rubra collected in natural grasslands of Western Spain. Methods Vis-NIR spectra (400-2498 nm) from 124 virus-infected and virus-free E. festucae isolates were recorded directly from ground and freeze-dried mycelium. To estimate how well the spectra for uninfected and infected fungal samples could be differentiated, we used partial least-squares discriminant analysis (PLS1-DA) and several data pre-treatments to develop calibration models. Results Applying the best regression model, obtained with two sampling years and using standard normal variate (SNV) combined with first derivative transformation to a new validating data set (42 samples), we obtained a correct classification for 75% of the uninfected isolates and up to 86% of the infected isolates. Conclusions The results obtained suggest that Vis-NIR spectroscopy is a promising technology for detection of viral infections in fungal samples when an alternative faster approach is desirable. It provides a tool adequately exact and more time- and cost-saving than the conventional reference analysis.
URIhttp://hdl.handle.net/10261/37668
Identifiershttp://dx.doi.org/10.1186/1743-422X-8-286
Appears in Collections:(IRNASA) Artículos
Files in This Item:
File Description SizeFormat 
1743-422X-8-286.xml57,98 kBXMLView/Open
1743-422X-8-286.pdf319,51 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.