English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/37617
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Comparative studies on lectin–carbohydrate interactions in low and high density homo- and heteroglycoclusters

AuthorsGómez García, Marta ; Benito, Juan M. ; Gutiérrez-Gallego, Ricardo; Maestre, Alfredo; Ortiz-Mellet, Carmen; García-Fernández, José Manuel; Jiménez Blanco, José L.
KeywordsCarbohydrates
Glycoconjugates
Surface Plasmon Resonance
Lectins
Concanavalin A
Molecular sequence data
Issue Date24-Feb-2010
PublisherRoyal Society of Chemistry
CitationOrganic and Biomolecular Chemistry 8(8): 1849-1860 (2010)
AbstractA versatile synthetic procedure to construct series of high- and low-density homo- and heteroglycoclusters is reported. The binding properties of these synthetic multivalent glycoconjugates to concanavalin A (Con A), a model lectin, have been assessed by using a range of competitive and non-competitive binding assays including enzyme-linked lectin assays (ELLA), isothermal titration microcalorimetry (ITC) and surface plasmon resonance (SPR). In all cases, highly dense glycoclusters showed a substantial amplification of the lectin-binding strength in comparison with low-density counterparts. Interestingly, highly-dense glycoligand presentations, regardless of their homo- or heteroglycoligand pattern, furnished similar Con A binding properties, supporting the existence of a synergic effect (heterocluster effect) due to secondary interactions of “non-active” structural motifs in the presence of a certain density of “active” glycoligands.
Description12 páginas, 5 figuras, 3 tablas, 3 esquemas.
Publisher version (URL)http://dx.doi.org/10.1039/B920048G
URIhttp://hdl.handle.net/10261/37617
DOI10.1039/B920048G
ISSN1177-0520
Appears in Collections:(IIQ) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.