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Título: | Comparative studies on lectin–carbohydrate interactions in low and high density homo- and heteroglycoclusters |
Autor: | Gómez García, Marta; Benito, Juan M. CSIC ORCID CVN ; Gutiérrez-Gallego, Ricardo; Maestre, Alfredo; Ortiz-Mellet, Carmen; García Fernández, José Manuel CSIC ORCID; Jiménez Blanco, José L. | Palabras clave: | Carbohydrates Glycoconjugates Surface Plasmon Resonance Lectins Concanavalin A Molecular sequence data |
Fecha de publicación: | 24-feb-2010 | Editor: | Royal Society of Chemistry (UK) | Citación: | Organic and Biomolecular Chemistry 8(8): 1849-1860 (2010) | Resumen: | A versatile synthetic procedure to construct series of high- and low-density homo- and heteroglycoclusters is reported. The binding properties of these synthetic multivalent glycoconjugates to concanavalin A (Con A), a model lectin, have been assessed by using a range of competitive and non-competitive binding assays including enzyme-linked lectin assays (ELLA), isothermal titration microcalorimetry (ITC) and surface plasmon resonance (SPR). In all cases, highly dense glycoclusters showed a substantial amplification of the lectin-binding strength in comparison with low-density counterparts. Interestingly, highly-dense glycoligand presentations, regardless of their homo- or heteroglycoligand pattern, furnished similar Con A binding properties, supporting the existence of a synergic effect (heterocluster effect) due to secondary interactions of “non-active” structural motifs in the presence of a certain density of “active” glycoligands. | Descripción: | 12 páginas, 5 figuras, 3 tablas, 3 esquemas. | Versión del editor: | http://dx.doi.org/10.1039/B920048G | URI: | http://hdl.handle.net/10261/37617 | DOI: | 10.1039/B920048G | ISSN: | 1177-0520 |
Aparece en las colecciones: | (IIQ) Artículos |
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