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dc.contributor.authorTanvig, Mette-
dc.contributor.authorBlaabjerg, Morten-
dc.contributor.authorAndersen, Rikke K.-
dc.contributor.authorVilla, Ana-
dc.contributor.authorRosager, Ann Mari-
dc.contributor.authorPoulsen, Frantz R.-
dc.contributor.authorMartínez-Serrano, Alberto-
dc.contributor.authorZimmer, Jens-
dc.contributor.authorMeyer, Morten-
dc.identifier.citationBrain Research 1295:1-12 (2009)es_ES
dc.description.abstractThe rostral migratory stream (RMS) is the main pathway by which newly born subventricular zone (SVZ) cells reach the olfactory bulb (OB) in rodents. This migration has been well studied in vivo, but an organotypic in vitro model would facilitate more experimental investigations. Here we introduce a slice culture preparation of the rat forebrain including en suite the rostral part of the lateral ventricle, the RMS and the OB. The preparation was validated with regard to endogenous cell proliferation and migration by tracking bromodeoxyuridine (BrdU)-labelled cells in newly established and 3 and 6 week old cultures. For testing the migratory abilities of exogenous precursor cells, rat SVZ neurospheres and human neural (HNS1 cells) and mesenchymal (hMSC-TERT) stem cell lines were micrografted to the rostral SVZ of 1 and 7 day old cultures. Two weeks later graft derivatives were identified by immunohistochemical staining for human nuclei (HNS1/hMSC-TERT cells) and BrdU (HNS1 cells/neurospheres). Numerous HNS1 cells and BrdU-positive neurosphere cells were found in the RMS. Having reached the OB, subpopulations of the cells expressed the astroglial markers glial fibrillary acidic protein/hAM and the neuronal markers NeuN/tyrosine hydroxylase. Interestingly, the hMSC-TERT cells remained at the implantation site, demonstrating a diversity in migratory capability of different precursor cells. In conclusion, the RMS in rat forebrain slice cultures retains its ability to support migration of endogenous and exogenous neural precursors, making the cultures highly feasible for studies of conditions and factors regulating cell migration.es_ES
dc.description.sponsorshipThis research was supported by the Danish MRC (J. No. 22-02-0150/22-02-0008), the Danish Centre for Stem Cell Research (J. No. 2052-01-0045) and as part of the European Science Foundation EUROCORES Programme EuroSTELLS, by funds from the European Commission Sixth Framework Programme (contract no. ERAS-CT-2003-980409). Work at the AMS group was supported by grants from the European Union (QLK3-CT-2001-02120), and Spanish Ministry of Science and Technology (MCYT SAF2001-1038-C02-02).es_ES
dc.subjectRostral migratory stream (RMS)es_ES
dc.subjectSlice culturees_ES
dc.subjectEndogenous celles_ES
dc.subjectExogenous precursores_ES
dc.subjectCell migrationes_ES
dc.subjectNeural stem celles_ES
dc.subjectOlfactory bulbes_ES
dc.subjectSubventricular zonees_ES
dc.subjectMesenchymal stem celles_ES
dc.titleA brain slice culture model for studies of endogenous and exogenous precursor cell migration in the rostral migratory stream.es_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderDanish Medical Research Council-
dc.contributor.funderDanish Centre for Stem Cell Research-
dc.contributor.funderEuropean Commission-
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)-
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