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Title

A brain slice culture model for studies of endogenous and exogenous precursor cell migration in the rostral migratory stream.

AuthorsTanvig, Mette; Blaabjerg, Morten; Andersen, Rikke K.; Villa, Ana; Rosager, Ann Mari; Poulsen, Frantz R.; Martínez-Serrano, Alberto ; Zimmer, Jens; Meyer, Morten
KeywordsRostral migratory stream (RMS)
Migration
Model
Slice culture
Rostral
Endogenous cell
Exogenous precursor
Cell migration
Neural stem cell
Forebrain
Olfactory bulb
Subventricular zone
Mesenchymal stem cell
Issue Date27-Oct-2009
PublisherElsevier
CitationBrain Research 1295:1-12 (2009)
AbstractThe rostral migratory stream (RMS) is the main pathway by which newly born subventricular zone (SVZ) cells reach the olfactory bulb (OB) in rodents. This migration has been well studied in vivo, but an organotypic in vitro model would facilitate more experimental investigations. Here we introduce a slice culture preparation of the rat forebrain including en suite the rostral part of the lateral ventricle, the RMS and the OB. The preparation was validated with regard to endogenous cell proliferation and migration by tracking bromodeoxyuridine (BrdU)-labelled cells in newly established and 3 and 6 week old cultures. For testing the migratory abilities of exogenous precursor cells, rat SVZ neurospheres and human neural (HNS1 cells) and mesenchymal (hMSC-TERT) stem cell lines were micrografted to the rostral SVZ of 1 and 7 day old cultures. Two weeks later graft derivatives were identified by immunohistochemical staining for human nuclei (HNS1/hMSC-TERT cells) and BrdU (HNS1 cells/neurospheres). Numerous HNS1 cells and BrdU-positive neurosphere cells were found in the RMS. Having reached the OB, subpopulations of the cells expressed the astroglial markers glial fibrillary acidic protein/hAM and the neuronal markers NeuN/tyrosine hydroxylase. Interestingly, the hMSC-TERT cells remained at the implantation site, demonstrating a diversity in migratory capability of different precursor cells. In conclusion, the RMS in rat forebrain slice cultures retains its ability to support migration of endogenous and exogenous neural precursors, making the cultures highly feasible for studies of conditions and factors regulating cell migration.
Publisher version (URL)http://dx.doi.org/10.1016/j.brainres.2009.07.075
URIhttp://hdl.handle.net/10261/37594
DOI10.1016/j.brainres.2009.07.075
ISSN0006-8993
Appears in Collections:(CBM) Artículos
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