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Título

Linkage map construction involving a reciprocal translocation

Autor Farré, A.; Lacasa-Benito, I.; Cistué Sola, Luis ; de Jong, J. H.; Romagosa, Ignacio; Jansen, J.
Fecha de publicación mar-2011
EditorSpringer
Citación Farré A, Lacasa I, Cistué L, de Jong JH, Romagosa I, Jansen J. Linkage map construction involving a reciprocal translocation. Theoretical and applied genetics 122 (5): 1029-1037 (2011)
ResumenThis paper is concerned with a novel statistical–genetic approach for the construction of linkage maps in populations obtained from reciprocal translocation heterozygotes of barley (Hordeum vulgare L.). Using standard linkage analysis, translocations usually lead to ‘pseudo-linkage’: the mixing up of markers from the chromosomes involved in the translocation into a single linkage group. Close to the translocation breakpoints recombination is severely suppressed and, as a consequence, ordering markers in those regions is not feasible. The novel strategy presented in this paper is based on (1) disentangling the “pseudo-linkage” using principal coordinate analysis, (2) separating individuals into translocated types and normal types and (3) separating markers into those close to and those more distant from the translocation breakpoints. The methods make use of a consensus map of the species involved. The final product consists of integrated linkage maps of the distal parts of the chromosomes involved in the translocation.
Descripción 33 Pag., 5 Fig. The definitive version is available at: http://www.springerlink.com/content/0040-5752/
Versión del editorhttp://dx.doi.org/10.1007/s00122-010-1507-2
URI http://hdl.handle.net/10261/37564
DOI10.1007/s00122-010-1507-2
ISSN0040-5752
E-ISSN1432-2242
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