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Título : Physiological responses and differential gene expression in Prunus rootstocks under iron deficiency conditions
Autor : Gonzalo Pascual, María José ; Moreno Sánchez, María Ángeles ; Gogorcena Aoiz, Yolanda
Palabras clave : Ferric chelate reductase
Iron chlorosis
roton extrusion
qRT-PCR
Fecha de publicación : jun-2011
Editor: Elsevier
Citación : Gonzalo MJ, Moreno MA. Physiological responses and differential gene expression in Prunus rootstocks under iron deficiency conditions. Journal of plant physiology 168 (9): 887-893 (2011)
Resumen: Two Prunus rootstocks, the Myrobalan plum P 2175 and the interspecific peach–almond hybrid, Felinem, were studied to characterize their biochemical and molecular responses induced under iron-Deficient conditions. Plants of both genotypes were submitted to different treatments using a hydroponic system that permitted removal of Fe from the nutrient solution. Control plants were grown in 90 μM Fe (III)-EDTA, Deficient plants were grown in an iron free solution, and plants submitted to an Inductor treatment were resupplied with 180 μM Fe (III)-EDTA over 1 and 2 days after a period of 4 or 15 days of growth on an iron-free solution. Felinem increased the activity of the iron chelate reductase (FC-R) in the Inductor treatment after 4 days of iron deprivation. In contrast, P 2175 did not show any response after at least 15 days without iron. The induction of the FC-R activity in this genotype was coincident in time with the medium acidification. These results suggest two different mechanisms of iron chlorosis tolerance in both Strategy I genotypes. Felinem would use the iron reduction as the main mechanism to capture the iron from the soil, and in P 2175, the mechanism of response would be slower and start with the acidification of the medium synchronized with the gradual loss of chlorophyll in leaves. To better understand the control of these responses at the molecular level, the differential expression of PFRO2, PIRT1 and PAHA2 genes involved in the reductase activity, the iron transport in roots, and the proton release, respectively, were analyzed. The expression of these genes, estimated by quantitative real-time PCR, was different between genotypes and among treatments. The results were in agreement with the physiological responses observed.
Descripción : 27 Pag., 5 Fig. The definitive version is available at: http://www.sciencedirect.com/science/journal/01761617
Versión del editor: http://dx.doi.org/10.1016/j.jplph.2010.11.017
URI : http://hdl.handle.net/10261/37555
DOI: 10.1016/j.jplph.2010.11.017
ISSN: 0176-1617
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