English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/37486
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Selective inhibition of beta-F1-ATPase mRNA translation in human tumours.

AuthorsWillers, Imke M. ; Isidoro, Antonio; Ortega, Álvaro D.; Fernández, Pedro L.; Cuezva, José M.
Keywordscancer
H+-ATP synthase
mitochondrion
oxidative phosphorylation
regulation of gene expression
translation
Issue Date24-Feb-2010
PublisherPortland Press
CitationBiochemical Journal 426: 319-326
AbstractDown-regulation of beta-F1-ATPase (the catalytic subunit of the mitochondrial H+-ATP synthase) is a hallmark of many human tumours. The expression level of beta-F1-ATPase provides a marker of the prognosis of cancer patients, as well as of the tumour response to chemotherapy. However, the mechanisms that participate in down-regulating its expression in human tumours remain unknown. In the present study, we have investigated the expression of beta-F1-ATPase mRNA (termed beta-mRNA) in breast, colon and lung adenocarcinomas and squamous carcinomas of the lung. Despite the down-regulation of the protein, tumour beta-mRNA levels remained either unchanged (breast and lung adenocarcinomas) or significantly increased (colon and squamous lung carcinomas) when compared with paired normal tissues, suggesting a specific translation-masking event for beta-mRNA in human cancer. Consistently, we show using cell-free translation assays that a large fraction (approximately 70%) of protein extracts derived from breast and lung adenocarcinomas specifically repress the translation of beta-mRNA. We show that the 3'UTR (3' untranslated region) of human beta-mRNA is a relevant cis-acting element required for efficient translation of the transcript. However, an RNA chimaera bearing the 3'UTR of human beta-mRNA does not recapitulate the inhibitory effect of tumour extracts on beta-mRNA translation. Overall, the findings of the present study support the hypothesis that down-regulation of the bioenergetic activity of mitochondria in human tumours is exerted by translation silencing of beta-mRNA.
Publisher version (URL)http://dx.doi.org/10.1042/BJ20091570
URIhttp://hdl.handle.net/10261/37486
DOI10.1042/BJ20091570
ISSN0264-6021
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
JMCuezva_BiochemJ_2010.pdf527,52 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.