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dc.contributor.authorRodríguez-Celma, Jorge-
dc.contributor.authorLattanzio, Giuseppe-
dc.contributor.authorGrusak, Michael A.-
dc.contributor.authorAbadía Bayona, Anunciación-
dc.contributor.authorAbadía Bayona, Javier-
dc.contributor.authorLópez-Millán, Ana Flor-
dc.identifier.citationRodríguez-Celma J, Lattanzio G, Grusak MA, Abadía A, Abadía J, López-Millán AF. Root Responses of Medicago truncatula Plants Grown in Two Different Iron Deficiency Conditions: Changes in Root Protein Profile and Riboflavin Biosynthesis. Journal of proteome research 10 (5): 2590-2601 (2011)es_ES
dc.description12 Pag., 1 Tabl., 6 Fig. The definitive version is available at: http://pubs.acs.org/journal/jprobses_ES
dc.description.abstractIron deficiency is a yield-limiting factor with major implications for field crop production in one-third of the world’s agricultural areas, especially those with high soil CaCO3. In the present work, a two-dimensional gel electrophoresis proteomic approach was combined with a study on the riboflavin synthesis pathway, including qPCR and riboflavin determination, to investigate Fe-deficiency responses in Medicago truncatula plants grown with and without CaCO3. Iron deficiency caused a de novo accumulation of DMRLs and GTPcII, proteins involved in riboflavin biosynthesis, as well as marked increases in root riboflavin concentrations and in the expression of four genes from the riboflavin biosynthetic pathway. Two novel changes found were the increased accumulation of proteins related to N recycling and protein catabolism. Other identified changes were consistent with previously found increases in glycolysis, TCA cycle, and stress-related processes. All effects were more marked in the presence of CaCO3. Our results show that the riboflavin biosynthesis pathway was up-regulated at the genomic, proteomic, and metabolomic levels under both Fe-deficiency treatments, especially in the presence of CaCO3. Results also indicate that N recycling occurs in M. truncatula upon Fe deficiency, possibly constituting an additional anaplerotic N and C source for the synthesis of secondary metabolites, carboxylates, and others.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Science and Innovation (MICINN; grants AGL2009- 09018 and AGL2010-16515, co-financed with FEDER), the trilateral Project Hot Iron (ERA- NET Plant Genome Research KKBE; MICINN EUI2008-03618), the Aragn Government (group A03), and the U.S. Department of Agriculture, Agricultural Research Service (under Agreement number 58-6250-0-008 to MAG). J.R.-C. was supported by an I3P-CSIC predoctoral fellowship.es_ES
dc.publisherAmerican Chemical Societyes_ES
dc.subjectCalcium carbonatees_ES
dc.subjectC/N metabolism;es_ES
dc.subjecttwo-dimensional gel electrophoresises_ES
dc.titleRoot Responses of Medicago truncatula Plants Grown in Two Different Iron Deficiency Conditions: Changes in Root Protein Profile and Riboflavin Biosynthesises_ES
dc.description.peerreviewedPeer reviewedes_ES
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