Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/36177
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Título : Regulation of the Stimulant Actions of Neurokinin A and Human Hemokinin-1 on the Human Uterus: A Comparison with Histamine
Autor : Pennefather, Jocelyn N., Patak, Eva, Ziccone, Sebastian, Lilley, Alison, Pinto Pérez, Francisco M., Page, Nigel M., Story, Margot E., Grover, Sonia, Candenas de Luján, M. Luz
Palabras clave : Eledoisin
Endokinin
Female reproductive tract
Hemokinin
Histamine
Human myometrium
Neprilysin
Neurokinin A
Neuropeptides
Pregnancy
Signal transduction
Tachykinins
Uterine contractions
Uterus
Fecha de publicación : 17-May-2006
Editor: Society for the Study of Reproduction
Resumen: Regulation of the contractile effects of tachykinins and histamine on the human uterus was investigated with biopsy sections of the outer myometrial layer. The effects of neurokinin A (NKA) and human hemokinin-1 (hHK-1) in tissues from pregnant but not from nonpregnant women were enhanced by the inhibition of neprilysin. The effects of NKA and eledoisin were blocked by the NK2 receptor antagonist SR 48968 but not by the NK1 receptor antagonist SR 140333 in tissues from both groups of women. Human HK-1 acted as a partial agonist blocked by SR 48968 and, to a lesser extent, by SR 140333; endokinin D was inactive. In tissues from pregnant women, responses to high potassium-containing Krebs solution were 2–3-fold higher than those from nonpregnant women. Mepyramine-sensitive maximal responses to histamine were similarly enhanced. The absolute maximum responses to NKA and its stable NK2 receptor-selective analogue, [Lys5MeLeu9Nle10]NKA(4–10), were increased in pregnancy, but their efficacies relative to potassium responses were decreased. Tachykinin potencies were lower in tissues from pregnant women than in those from nonpregnant women. These data 1) show for the first time that hHK-1 is a uterine stimulant in the human, 2) confirm that the NK2 receptor is predominant in mediating tachykinin actions on the human myometrium, and 3) indicate that mammalian tachykinin effects are tightly regulated during pregnancy in a manner that would negate an inappropriate uterotonic effect. The potencies of these peptides in tissues from nonpregnant women undergoing hysterectomy are consistent with their possible role in menstrual and menopausal disorders.
Descripción : 8 páginas, 7 figuras, 1 tabla
Versión del editor: http://dx.doi.org/10.1095/​biolreprod.106.051508
URI : http://hdl.handle.net/10261/36177
ISSN: 0006-3363
DOI: 10.1095/​biolreprod.106.051508
Citación : Biology of Reproduction 75(3): 334-341 (2006)
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