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Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system

AuthorsSanz, Libia ; Bazaa, Amine; Marrakchi, Naziha; Pérez, Alicia ; Chenik, Mehdi; Bel Lasfer, Zakaria; El Ayeb, Mohamed; Calvete, Juan J.
KeywordsCerastes vipera
Macrovipera lebetina transmediterranea
Integrin inhibition
Snake venom disintegrin
Snake venomics
Toxin evolution
Issue Date28-Mar-2006
PublisherBiochemical Society
CitationBiochem J. 2006 April 15; 395(Pt 2): 385–392
AbstractWe report the cloning and sequence analysis of Cerastes vipera and Macrovipera lebetina transmediterranea cDNAs coding for short non-RGD (Arg-Gly-Asp) disintegrins and for dimeric disintegrin subunits. The mRNAs belong to the short-coding class, suggesting that these disintegrin mRNAs may be more widely distributed than previously thought. Our data also argue for a common ancestry of the mRNAs of short disintegrins and those coding for precursors of dimeric disintegrin chains. The Macrovipera lebetina transmediterranea dimeric disintegrin reported to inhibit the laminin-binding integrins α3β1, α6β1 and α7β1 was analysed using a proteomic approach and was shown to bear MLD (Met-Leu-Asp) and VGD (Val-Gly-Asp) motifs. The results highlight the fact that disintegrins have evolved a restricted panel of integrin-blocking sequences that segregate with defined branches of the phylogenetic tree of the integrin α-chains, providing novel insights into the evolutionary adaptation of the snake venom antagonists to the ligand-binding sites of their target integrin receptors.
DescriptionCopyright © by Portland Press. The final version of record is available at http://www.biochemj.org/bj/default.htm
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