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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/35599
Title: An evolutionarily conserved three-dimensional structure in the vertebrate Irx clusters facilitates enhancer sharing and coregulation
Authors: Tena, Juan J.; Alonso, M. Eva; Calle-Mustienes, Elisa de la; Splinter, Erik; Laat, Wouter de; Manzanares, Miguel; Gómez-Skarmeta, José Luis
Keywords: Biological Sciences
Developmental biology
Issue Date: 10-May-2011
Publisher: Nature Publishing Group
Citation: Nature Communications 2 : 310 (2011)
Abstract: Developmental gene clusters are paradigms for the study of gene regulation; however, the mechanisms that mediate phenomena such as coregulation and enhancer sharing remain largely elusive. Here we address this issue by analysing the vertebrate Irx clusters. We first present a deep enhancer screen of a 2-Mbp span covering the IrxA cluster. Using chromosome conformation capture, we show that enhancer sharing is widespread within the cluster, explaining its evolutionarily conserved organization. We also identify a three-dimensional architecture, probably formed through interactions with CCCTC-binding factor, which is present within both Irx clusters of mouse, Xenopus and zebrafish. This architecture brings the promoters of the first two genes together in the same chromatin landscape. We propose that this unique and evolutionarily conserved genomic architecture of the vertebrate Irx clusters is essential for the coregulation of the first two genes and simultaneously maintains the third gene in a partially independent regulatory landscape.
Publisher version (URL): http://dx.doi.org/10.1038/ncomms1301
URI: http://hdl.handle.net/10261/35599
DOI: 10.1038/ncomms1301
E-ISSN: 2041-1723
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