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1openAccesscatalysts-14-00115.pdf.jpgfeb-2024Tuning Almond Lipase Features by Using Different Immobilization SupportsCherni, Oumaima; Carballares, Diego; Siar, El Hocine; Abellanas-Pérez, Pedro; Andrades, Diandra de; Rocha-Martín, Javier CSIC ORCID; Bahri, Sellema; Fernández-Lafuente, Roberto CSIC ORCIDartículo
2closedAccessaccesoRestringido.pdf.jpgago-2015Production and immobilization of Geotrichum candidum lipase via physical adsorption on eco-friendly support: Characterization of the catalytic properties in hydrolysis and esterification reactionsRamos, Elisa Z.; Júnior, R.H.M.; Castro, Patricia F. de; Tardioli, Paulo W.; Mendes, Adriano A.; Fernández-Lafuente, Roberto CSIC ORCID; Hirata, Daniela Bartículo
3openAccesscatalysts-08-00511.pdf.jpg2-nov-2018Immobilization of Eversa Lipase on Octyl Agarose Beads and Preliminary Characterization of Stability and Activity FeaturesArana-Peña, Sara; Lokha, Yuliya; Fernández-Lafuente, Roberto CSIC ORCIDartículo
4openAccessijms-20-04018.pdf.jpg17-ago-2019Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticlesMonteiro, Rodolpho R. C.; Lima, Paula J. M.; Pinheiro, Bruna B.; Freire, Tiago M.; Dutra, Lillian M. U.; Fechine, Pierre B. A.; Gonçalves, Luciana R. B.; de Souza, Maria C. M.; Santos, José C. S. dos; Fernández-Lafuente, Roberto CSIC ORCIDartículo
5closedAccessaccesoRestringido.pdf.jpgsep-2015Tuning the catalytic properties of lipases immobilized on divinylsulfone activated agarose by altering its nanoenvironmentSousa dos Santos, José Cleiton; Rueda, Nazzoly; Gonçalves, Luciana R. B.; Fernández-Lafuente, Roberto CSIC ORCIDartículo
6closedAccessaccesoRestringido.pdf.jpgjun-2015Evaluation of divinylsulfone activated agarose to immobilize lipases and to tune their catalytic propertiesSousa dos Santos, José Cleiton; Rueda, Nazzoly; Torres Sáez, Rodrigo; Barbosa, Oveimar CSIC ORCID; Gonçalves, Luciana R. B.; Fernández-Lafuente, Roberto CSIC ORCIDartículo
7openAccessDal_Magro_Immobilization_Biotechnol Rep_Amst_2019.pdf.jpg2019Immobilization of pectinase on chitosan-magnetic particles: Influence of particle preparation protocol on enzyme properties for fruit juice clarificationDal-Magro,Lucas; Silva de Moura, Kelly; Backes, Betina Elys; de-Menezes,Eliana Weber; Benvenutti, Edilson Valmir; Nicolodi, Sabrina; Klein, Manuela P.; Fernández-Lafuente, Roberto CSIC ORCID; Rodrigues, Rafael C.artículo
8openAccessmolecules-22-00601.pdf.jpg8-abr-2017Special Issue: Enzyme Immobilization 2016Fernández-Lafuente, Roberto CSIC ORCIDartículo
9closedAccess8-ene-2007Improved Stabilization of Genetically Modified Penicillin G Acylase in the Presence of Organic Cosolvents by Co- Immobilization of the Enzyme with PolyethyleneimineMontes, Tamara CSIC; Grazú, Valeria CSIC ORCID; Manso, Isabel CSIC; Galán, Beatriz CSIC ORCID ; López-Gallego, Fernando CSIC ORCID; González García, Ramón CSIC ORCID ; Hermoso, Juan A. CSIC ORCID; García, José Luis CSIC ORCID ; Guisán, José Manuel CSIC ORCID ; Fernández-Lafuente, Roberto CSIC ORCIDartículo
10closedAccessaccesoRestringido.pdf.jpg10-abr-2013Stabilization of the hexameric glutamate dehydrogenase from Escherichia coli by cations and polyethyleneimineGarcía-Galán, Cristina; Barbosa, Oveimar CSIC ORCID; Fernández-Lafuente, Roberto CSIC ORCIDartículo
11closedAccessaccesoRestringido.pdf.jpg2010Two step ethanolysis: a simple and efficient way to improve the enzymatic biodiesel synthesis catalyzed by an immobilized-stabilized lipase from Thermomyces lanuginosusRodrigues, Rafael C.; Pessela, Benevides C. CSIC ORCID ; Volpato, Giandra CSIC; Fernández-Lafuente, Roberto CSIC ORCID; Guisán, José Manuel CSIC ORCID artículo
12openAccessmolecules-23-02993.pdf.jpg16-nov-2018Evaluation of strategies to produce highly porous cross-linked aggregates of porcine pancreas lipase with magnetic propertiesGuimarães, José Renato; Giordano, Raquel de Lima Camargo; Fernández-Lafuente, Roberto CSIC ORCID; Tardioli, Paulo W.artículo
13openAccessComposites_Passos_Art2020.pdf.jpg2020Composites of Crosslinked Aggregates of Eversa® Transform and Magnetic Nanoparticles. Performance in the Ethanolysis of Soybean OilPassos Miranda, Letícia; Guimarães, José Renato; Campos Giordano, Roberto; Fernández-Lafuente, Roberto CSIC ORCID; Tardioli, Paulo W.artículo
14openAccessThe_Immobilization_and_Stabilization_Miguez.pdf.jpg4-oct-2023The Immobilization and Stabilization of Trypsin from the Porcine Pancreas on Chitosan and Its Catalytic Performance in Protein HydrolysisMiguez, João Pedro; Fernández-Lafuente, Roberto CSIC ORCID; Tavano, Olga Luisa; Mendes, Adriano A.artículo
15openAccessmolecules-21-01577.pdf.jpg19-nov-2016Agarose and Its Derivatives as Supports for Enzyme ImmobilizationZucca, Paolo; Fernández-Lafuente, Roberto CSIC ORCID; Sanjust, Enricoartículo
16openAccessmolecules-23-03188-v2.pdf.jpg3-dic-2018Further stabilization of alcalase immobilized on glyoxyl supports: amination plus modification with glutaraldehydeHussain, Fouzia; Arana-Peña, Sara; Morellon-Sterling, Roberto; Barbosa, Oveimar CSIC ORCID; Ait Braham, Sabrina; Kamal, Shagufta; Fernández-Lafuente, Roberto CSIC ORCIDartículo
17openAccessWO_2003_070928_A1.pdf.jpg28-ago-2003Hydrolysis of lactose with immobilised thermoresistant lactase and the production method thereofPessela, Benevides C. CSIC ORCID ; Vian Herrero, Alejandro; Guisán, José Manuel CSIC ORCID ; Carrascosa, Alfonso V. CSIC ORCID ; Fernández-Lafuente, Roberto CSIC ORCID; Mateo Ruiz, César; García, José Luis CSIC ORCID patente
18openAccessethyl_butyrate_synthesis_catalyzed_lipases_A_B_candida_antarctica_immobilized_magnetic_nanoparticles.pdf.jpg19-nov-2019Ethyl butyrate synthesis catalyzed by lipases A and B from candida antarctica immobilized onto magnetic nanoparticles. Improvement of biocatalysts’ performance under ultrasonic irradiationMonteiro, Rodolpho R. C.; Neto, Davino M. Andrade; Fechine, Pierre B. A.; Lopes, Ada A. S.; Gonçalves, Luciana R. B.; Dos Santos, José C. S.; de Souza, Maria C. M.; Fernández-Lafuente, Roberto CSIC ORCIDartículo
19openAccesscatalysts-09-00487-v2.pdf.jpg27-may-2019Reuse of lipase from pseudomonas fluorescens via its step-by-step coimmobilization on glyoxyl-octyl agarose beads with least stable lipasesRios, Nathalia S.; Arana-Peña, Sara; Mendez-Sanchez, Carmen; Ortiz, Claudia; Gonçalves, Luciana R. B.; Fernández-Lafuente, Roberto CSIC ORCIDartículo
20openAccessGlutaraldehyde_Barbosa_Publisherversion2014.pdf.jpg2014Glutaraldehyde in bio-catalysts design: A useful crosslinker and a versatile tool in enzyme immobilizationBarbosa, Oveimar CSIC ORCID; Ortiz, Claudia; Berenguer Murcia, A.; Torres Sáez, Rodrigo; Rodrigues, Rafael C.; Fernández-Lafuente, Roberto CSIC ORCIDartículo
21openAccessReactivation_Rueda_Art2015.pdf.jpg2015Reactivation of lipases by the unfolding and refolding of covalently immobilized biocatalystsRueda, Nazzoly; Sousa dos Santos, José Cleiton; Torres Sáez, Rodrigo; Barbosa, Oveimar CSIC ORCID; Ortiz, Claudia; Fernández-Lafuente, Roberto CSIC ORCIDartículo
22openAccesscatalysts-13-00223.pdf.jpg18-ene-2023Technical-Economic Assessment-The Missing Piece for Increasing the Attractiveness of Applied Biocatalysis in Ester Syntheses?Sousa, Ronaldo Rodrigues de; Castro, Rui de Paula Vieira de; Assis, Nadinne Medeiros; Silva, Ayla Sant’Ana da; Freire, Denise Maria Guimarães; Fernández-Lafuente, Roberto CSIC ORCID; Ferreira-Leitão, Viridiana Santanaartículo
23openAccessmolecules-26-00193-v2.pdf.jpg2-ene-2021Immobilization of Eversa® Transform via CLEA Technology Converts It in a Suitable Biocatalyst for Biolubricant Production Using Waste Cooking OilGuimarães, José Renato; Passos Miranda, Letícia; Fernández-Lafuente, Roberto CSIC ORCID; Tardioli, Paulo Waldirartículo
24closedAccesssep-1999Stabilization of multimeric enzymes via immobilization and post-immobilization techniquesFernández-Lafuente, Roberto CSIC ORCID; Rodríguez, Verónica; Mateo González, César CSIC ORCID CVN; Penzol, Guadalupe; Guisán, José Manuel CSIC ORCID artículo
25closedAccessaccesoRestringido.pdf.jpgago-2013Characterization of the catalytic properties of lipases from plant seeds for the production of concentrated fatty acids from different vegetable oilsSantos, Kádima C.; Cassimiro, Débora M.J.; Avelar, Matheus H.M; Hirata, Daniela B.; Castro, Heizir F. de; Fernández-Lafuente, Roberto CSIC ORCID; Mendes, Adriano A.artículo
26closedAccessaccesoRestringido.pdf.jpgjul-2014Comparison of the performance of commercial immobilized lipases in the synthesis of different flavor estersMartins, Andrea B.; Silva, Alexandre M.; Schein, Mirela F.; García-Galán, Cristina; Záchia Ayub, Marco A.; Fernández-Lafuente, Roberto CSIC ORCID; Rodrigues, Rafael C.artículo
27closedAccessaccesoRestringido.pdf.jpg25-ene-2015Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipasesPoppe, Jakeline K.; Matte, Carla R.; Ruaro Peralba, Maria do Carmo; Fernández-Lafuente, Roberto CSIC ORCID; Rodrigues, Rafael C.; Záchia Ayub, Marco A.artículo
28openAccessImproved_Rueda_Art2015.pdf.jpg2015Improved performance of lipases immobilized on heterofunctional octyl-glyoxyl agarose beadsRueda, Nazzoly; Sousa dos Santos, José Cleiton; Torres Sáez, Rodrigo; Ortiz, Claudia; Barbosa, Oveimar CSIC ORCID; Fernández-Lafuente, Roberto CSIC ORCIDartículo
29closedAccessaccesoRestringido.pdf.jpgsep-2014Stabilizing hyperactivated lecitase structures through physical treatment with ionic polymersDos Santos, José C. S.; García-Galán, Cristina; Rodrigues, Rafael C.; Batista de Sant’Ana, Hosiberto; Gonçalves, Luciana R. B.; Fernández-Lafuente, Roberto CSIC ORCIDartículo
30openAccessVersatility_Santos_Art2015.pdf.jpg2015Versatility of divinylsulfone supports permits the tuning of CALB properties during its immobilizationSousa dos Santos, José Cleiton; Rueda, Nazzoly; Sánchez, Alfredo; Gonçalves, Luciana R. B.; Fernández-Lafuente, Roberto CSIC ORCIDartículo
31openAccessAmination_Rodrigues_2014.pdf.jpg2014Amination of enzymes to improve biocatalyst performance: Coupling genetic modification and physicochemical toolsRodrigues, Rafael C.; Barbosa, Oveimar CSIC ORCID; Ortiz, Claudia; Berenguer Murcia, A.; Torres Sáez, Rodrigo; Fernández-Lafuente, Roberto CSIC ORCIDartículo
32openAccessStyrene-Divinylbenzene_Beads_Garcia-Galan.pdf.jpg10-jun-2014Evaluation of styrene-divinylbenzene beads as a support to immobilize lipasesGarcía-Galán, Cristina; Barbosa, Oveimar CSIC ORCID; Hernández Sánchez, Karel CSIC ORCID; Dos Santos, José C. S.; Rodrigues, Rafael C.; Fernández-Lafuente, Roberto CSIC ORCIDartículo
33openAccessGlyoxyl-Activated_Antunes_Art2020.pdf.jpg2020Glyoxyl-Activated Agarose as Support for Covalently Link Novo-Pro D: Biocatalysts Performance in the Hydrolysis of CaseinAntunes Lopes, Laiane; Kern Novelli, Paula; Fernández-Lafuente, Roberto CSIC ORCID; Waldir Tardioli, Paulo; Camargo Giordano, Raquel Limaartículo
34openAccessDiaz_Ramos_Improving_molecules-24-04392.pdf.jpg1-dic-2019Improving the Yields and Reaction Rate in the Ethanolysis of Soybean Oil by Using Mixtures of Lipase CLEAsDíaz Ramos, Margarita; Passos Miranda, Letícia; Fernández-Lafuente, Roberto CSIC ORCID; Kopp, William; Tardioli, Paulo Waldirartículo
35closedAccessaccesoRestringido.pdf.jpgjun-2013Effect of immobilization protocol on optimal conditions of ethyl butyrate synthesis catalyzed by lipase B from Candida antarcticaFriedrich, John L. R.; Peña, Fernanda P.; García-Galán, Cristina; Fernández-Lafuente, Roberto CSIC ORCID; Ayub, Marco A. Z.; Rodrigues, Rafael C.artículo
36openAccesscatalysts-09-00657-v3.pdf.jpg31-jul-2019Combi-CLEAs of glucose oxidase and catalase for conversion of glucose to gluconic acid eliminating the hydrogen peroxide to maintain enzyme activity in a bubble column reactorOliveira Mafra, Agnes Cristina; Gazzotto Ulrich, Letícia; Kornecki, Jakub F.; Fernández-Lafuente, Roberto CSIC ORCID; Tardioli, Paulo W.; Perencin de Arruda Ribeiro, Marceloartículo
37openAccessmolecules-22-00091.pdf.jpg5-ene-2017Desorption of Lipases Immobilized on Octyl-Agarose Beads and Coated with Ionic Polymers after Thermal Inactivation. Stronger Adsorption of Polymers/Unfolded Protein CompositesVirgen-Ortíz, José J.; Pedrero, Sara G.; Fernández-López, Laura; López-Carrobles, Nerea; Gorines, Beatriz C.; Otero Hernández, Cristina; Fernández-Lafuente, Roberto CSIC ORCIDartículo
38openAccessSustainable_Mendoza_Art2020.pdf.jpg2020Sustainable Enzymatic Synthesis of a Solketal Ester—Process Optimization and Evaluation of Its Antimicrobial ActivityMendoza-Ortiz, Patricia A.; Gama, Rafaela S.; Gómez, Omar C.; Luiz, Jaine H. H.; Fernández-Lafuente, Roberto CSIC ORCID; Cren, Erika C.; Mendes, Adriano A.artículo
39openAccessStabilizing_Fernández-López_Art2015.pdf.jpg2015Stabilizing effects of cations on lipases depend on the immobilization protocolFernández-López, Laura; Bartolomé-Cabrero, Rocío CSIC; Rodriguez, María Daniela; Sousa dos Santos, José Cleiton; Rueda, Nazzoly; Fernández-Lafuente, Roberto CSIC ORCIDartículo
40openAccess8-dic-2014Enzyme immobilization, an old-fashioned tool or a modern strategy to improve enzyme properties?Fernández-Lafuente, Roberto CSIC ORCIDartículo
41openAccessjodeiri-et-al-additively-ACS_Appl_Mater_Interfaces 2023_vs_pub.pdf.jpg10-mar-2023Additively Manufactured 3D Micro-bioelectrodes for Enhanced Bioelectrocatalytic OperationJodeiri, Keyvan; Foerster, Aleksandra; Trindade, Gustavo F.; Im, Jisun; Carballares, Diego; Fernández-Lafuente, Roberto CSIC ORCID; Pita, Marcos CSIC ORCID ; López de Lacey, Antonio CSIC ORCID ; Tuck, Christopherartículo
42closedAccessfeb-2004Stabilization of Penicillin G Acylase from Escherichia coli: Site-Directed Mutagenesis of the Protein Surface To Increase Multipoint Covalent AttachmentAbian, Olga CSIC ORCID; Grazú, Valeria CSIC ORCID; Hermoso, Juan A. CSIC ORCID; González García, Ramón CSIC ORCID ; García, José Luis CSIC ORCID ; Fernández-Lafuente, Roberto CSIC ORCID; Guisán, José Manuel CSIC ORCID artículo
43openAccessEnzyme_Immobilization_Fernandez-Lafuente.pdf.jpg10-dic-2014Editorial: Special issue - Enzyme immobilizationFernández-Lafuente, Roberto CSIC ORCIDartículo
44closedAccess13-feb-2009Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangementsCarrasco-López, César CSIC ORCID; Godoy, César A. CSIC ORCID; Rivas, Blanca de las CSIC ORCID; Fernández-Lorente, Gloria CSIC ORCID ; Palomo, José Miguel; Guisán, José Manuel CSIC ORCID ; Fernández-Lafuente, Roberto CSIC ORCID; Martínez-Ripoll, Martín CSIC ORCID; Hermoso, Juan A. CSIC ORCIDartículo
45openAccessmolecules-28-01373.pdf.jpg1-feb-2023Enzyme ImmobilizationFernández-Lafuente, Roberto CSIC ORCIDeditorial
46openAccesscatalysts-08-00496.pdf.jpg26-oct-2018Preparation of Magnetic Cross-Linked Amyloglucosidase Aggregates: Solving Some Activity ProblemsAmaral-Fonseca, Murilo; Kopp, Willian; Giordano, R. L. C.; Fernández-Lafuente, Roberto CSIC ORCID; Tardioli, Paulo W.artículo
47closedAccessaccesoRestringido.pdf.jpgdic-2013Optimized butyl butyrate synthesis catalyzed by thermomyces lanuginosus lipaseMartins, Andrea B.; Friedrich, B.; Rodrigues, Rafael C.; García-Galán, Cristina; Fernández-Lafuente, Roberto CSIC ORCID; Ayub, Marco A. Z.artículo
48closedAccessaccesoRestringido.pdf.jpg17-ago-2015Importance of the Support Properties for Immobilization or Purification of EnzymesSousa dos Santos, José Cleiton; Barbosa, Oveimar CSIC ORCID; Ortiz, Claudia; Berenguer Murcia, A.; Rodrigues, Rafael C.; Fernández-Lafuente, Roberto CSIC ORCIDartículo
49openAccesscatalysts-07-00009.pdf.jpg1-ene-2017The Protagonism of Biocatalysis in Green Chemistry and Its Environmental BenefitsFerreira-Leitão, Viridiana Santana; Cammarota, Magali Christe; Gonçalves Aguieiras, Erika Cristina; Vasconcelos de Sá, Lívian Ribeiro; Fernández-Lafuente, Roberto CSIC ORCID; Freire, Denise Maria Guimarãesartículo
50closedAccessaccesoRestringido.pdf.jpgoct-2015Strategies for the one-step immobilization-purification of enzymes as industrial biocatalystsBarbosa, Oveimar CSIC ORCID; Ortiz, Claudia; Berenguer Murcia, A.; Torres Sáez, Rodrigo; Rodrigues, Rafael C.; Fernández-Lafuente, Roberto CSIC ORCIDartículo
Resultados 1-50 de 98.