Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/35223
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Vendrell, Iolanda | - |
dc.contributor.author | Carrascal, Montserrat | - |
dc.contributor.author | Campos, Francisco | - |
dc.contributor.author | Abián, Joaquín | - |
dc.contributor.author | Suñol, Cristina | - |
dc.date.accessioned | 2011-05-03T12:58:40Z | - |
dc.date.available | 2011-05-03T12:58:40Z | - |
dc.date.issued | 2010-01-01 | - |
dc.identifier.citation | Toxicology and Applied Pharmacology 242(1): 109-118 (2010) | es_ES |
dc.identifier.issn | 0041-008X | - |
dc.identifier.uri | http://hdl.handle.net/10261/35223 | - |
dc.description.abstract | Methylmercury is an environmental contaminant that is particularly toxic to the developing central nervous system; cerebellar granule neurons are especially vulnerable. Here, primary cultures of cerebellar granule cells (CGCs) were continuously exposed to methylmercury for up to 16 days in vitro (div). LC50 values were 508 ± 199, 345 ± 47, and 243 ± 45 nM after exposure for 6, 11, and 16 div, respectively. Proteins from cultured mouse CGCs were separated by 2DE. Seventy-one protein spots were identified by MALDI-TOF PMF and MALDI-TOF/TOF sequencing. Prolonged exposure to a subcytotoxic concentration of methylmercury significantly increased non-phosphorylated cofilin both in cell protein extracts (1.4-fold; p < 0.01) and in mitochondrial-enriched fractions (1.7-fold; p < 0.01). The decrease in P-cofilin induced by methylmercury was concentration-dependent and occurred after different exposure times. The percentage of P-cofilin relative to total cofilin significantly decreased to 49 ± 13% vs. control cells after exposure to 300 nM methylmercury for 5 div. The balance between the phosphorylated and non-phosphorylated form of cofilin regulates actin dynamics and facilitates actin filament turnover. Filamentous actin dynamics and reorganization are responsible of neuron shape change, migration, polarity formation, regulation of synaptic structures and function, and cell apoptosis. An alteration of the complex regulation of the cofilin phosphorylation/dephosphorylation pathway could be envisaged as an underlying mechanism compatible with reported signs of methylmercury-induced neurotoxicity. | es_ES |
dc.description.sponsorship | This research was supported by grants FIS-PI061212 and 2005/SGR/00826. The LP CSIC/UAB is member of ProteoRed, funded by Genoma Spain, and follows the quality criteria set up by ProteoRed standards. I. Vendrell had a CSIC fellowship in the I3P program cofinanced with European Social Funds. | - |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | Neuroproteome | es_ES |
dc.subject | Cerebellar granule neurons | es_ES |
dc.subject | Methylmercury | es_ES |
dc.subject | Neurotoxicity | es_ES |
dc.subject | Cofilin | es_ES |
dc.subject | In vitro | es_ES |
dc.title | Methylmercury disrupts the balance between phosphorylated and non-phosphorylated cofilin in primary cultures of mice cerebellar granule cells: a proteomic study | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.taap.2009.09.022 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.taap.2009.09.022 | es_ES |
dc.identifier.e-issn | 1096-0333 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (IIBB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
38
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
32
checked on 25-feb-2024
Page view(s)
345
checked on 24-abr-2024
Download(s)
124
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.